Transcription profiling of Entamoeba histolytica clones derived from the same isolate but with different pathogenic properties
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ABSTRACT: The availability of two genetically very similar clones (A and B) derived from the laboratory isolate Entamoeba histolytica HM-1:IMSS, which differ in their virulence properties, provides a powerful tool for identifying pathogenicity factors of the causative agent of human amoebiasis. We isolated RNA from each clone for transcriptional comparison.
Project description:This experiment probed for the presence of known Arabidopsis and rice microRNAs in total RNA samples derived from species representative of the major groups of land plants: Eudicots (Arabidopsis thaliana, Nicotiana benthamiana), monocots (Oryza satica, Triticum aestivum), magnoliids (Liriodendron tulipifera), gymnosperms (Pinus resinosa), ferns (Ceratopteris thalictroides), lycopods (Selaginella uncinata), and mosses (Polytrichum juniperinum). In most cases two technical or biological replicates were performed.
Project description:Each category of Met4 cofactor(s) was systematically deleted and the strains were analyzed for genome-wide transcription at different times following induction of hyperactive Met4.
Project description:All yeast strains used in this study (Table 1) are in the W303 background (ade2-1 can1-100, his3-1,15 leu2-3,112 trp1-1 ura3). For sulfur limitation microarray studies, WT, met4 delete, met31 delete met32 delete, cbf1 delete, and met28 delete strains were grown in minimal B-media [see Cherest, H., and Surdin-Kerjan, Y. (1992). Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway. Genetics 130, p51-58 for B-media composition] supplemented with 0.5mM methionine as the sole sulfur source. An aliquot of cells was harvested for a t=0 time point while the remainder were filtered through a .22um Stericup filter (Millipore), then washed and resuspended in pre-warmed (30 C) B-media lacking any source of sulfur. Cells were harvested after 20, 40, and 80 minutes.
Project description:This experiment was designed to determine the presence and levels of microRNAs and other select small RNAs in the major organs of wild-type (Col-0) Arabidopsis thaliana grown under standard conditions. <br><br><br><br>To obtain a global overview of miRNA levels in wild-type Arabidopsis, a tissue expression map was generated. Two samples of total RNA from siliques, stems, cauline leaves, roots, short-day seedlings, long-day seedlings, and rosette leaves as well as four samples from inflorescences were obtained. All duplicate samples were harvested from independent crops of plants grown under the same conditions, and thus represent biologcial replicates. Hybridizations were always comparing a given sample against a common, synthetic reference set. 225 reference DNA oligos comprising the sense strand of each RNA represented on the array flanked by constant 5' and 3' sequences representing the adapters used in RNA cloning (Axtell and Bartel). Amplification and Cy5 end-labeling of this synthetic set of oligos provides a constant reference signal that allows comparison of different Cy3-labeled biological samples.
Project description:Chromatin immunoprecipitation and hybridization to a chromosome-wide DNA tiling array (ChIP-chip)was performed to compare the distribution pattern of H3K9me2 between nrpd1a-4_nrpd1b-11 and wild type. Experiments were done using two independent biological replicates.<br>
Project description:Chromatin immunoprecipitation and hybridization to a chromosome-wide DNA tiling array (ChIP-chip)was performed to compare the distribution of H3K4me2 in nrpd1a-4_nrpd1b-11 double mutant and in the wild type. Experiments were done using two independent biological replicates.<br><br>
Project description:Effects of furanone on global gene expression. CD-1mice were treated with compond A B or C for 21 days. The compoud was administered orally to each mouse using a gastric sonde.The researcher was not previously informed about wich compound was the control, this to ensure impartiality during the experiment and the outcoume of the results.