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Legionella pneumophila transcriptomic response to CuO nanoparticles

ABSTRACT: Legionella pneumophila was exposed for 3h to 160 ug/ml of CuO nanoparticle. Untreated samples are used as the control condition.

ORGANISM(S): Legionella pneumophila subsp. pneumophila str. Philadelphia 1 Legionella pneumophila

PROVIDER: GSE41966 | GEO | 2013/03/04

SECONDARY ACCESSION(S): PRJNA178624

REPOSITORIES: GEO

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Legionella pneumophila transcriptomic response to CuO nanoparticles

Project description:Legionella pneumophila was exposed for 3h to 160 ug/ml of CuO nanoparticle. Untreated samples are used as the control condition. Two conditions: CuO (treated) and UN (untreated control. Three bioilogical repliucates. Genomic DNA is used as a reference channel. (Talaat, 2002, NAR).

2013-03-04 | E-GEOD-41966 | biostudies-arrayexpress

Legionella pneumophila

Project description:Legionella pneumophila transcriptomic response to CuO nanoparticles

| PRJNA178624 | ENA

The effect of CuO nanoparticles on gene expression patterns in Daphnia magna

Project description:There is still a lot of contradiction on whether metal ions are solely responsible for the observed the toxicity of ZnO and CuO nanoparticles to aquatic species. While most tests have studied nanoparticle effects at organismal levels (e.g. mortality, reproduction), effects at suborganismal levels may clarify the role of metal ions, nanoparticles and nanoparticle aggregates. In this study, the effect of ZnO, CuO nanoparticles and zinc, copper salts was tested on the gene expression levels in Daphnia magna. D. magna was exposed during 96 hours to 10% immobilization concentrations of all chemicals, after which daphnids were sampled for a differential gene expression analysis using microarray. When comparing the nanoparticle exposed daphnids (ZnO or CuO) to the metal salt exposed daphnids (zinc or copper salt), the microarray results showed no significantly differentially expressed genes. These results indicate that the toxicity of the tested ZnO and CuO nanoparticles to D. magna caused is solely caused by toxic metal ions. 4 replicate exposures of ZnO nanoparticles, ZnCl2, Blank (for Zn); 4 replicate exposures of CuO nanoparticles, CuCl2.2H2O, Blank (for Cu); Individual reference design with swapped dyes for zinc (e.g. ZnO-REFZn; REFZn-bl) and copper exposure (e.g. CuO-REFCu; REFCu-bl); Zinc reference sample is a mixture of equal aliquots of ZnO nanoparticle, ZnCl2 and blank; Copper reference sample is a mixture of equal aliquots of CuO nanoparticle, CuCl2.2H2O and blank

2014-03-01 | E-GEOD-54738 | biostudies-arrayexpress

Legionella pneumophila proteome

Project description:Legionella pneumophila cells were harvested during exponential growth (RP) and stationary growth (TP). VBNC cells were also anylzed. Protein subfractions were studied.

2015-11-13 | PXD001926 | Pride

The effect of CuO nanoparticles on gene expression patterns in Daphnia magna

2014-03-01 | GSE54738 | GEO

Legionella pneumophila

Project description:Legionella pneumophila

| PRJNA526503 | ENA

Legionella pneumophila transcriptomic response of hfq mutant during post-exponential phase

Project description:Legionella pneumophila wild-type and hfq mutant were grown to post-exponential. The wild-type strain is used as the control.

2013-05-21 | GSE42905 | GEO

small RNA profiling of human blood derived macrophages upon infection with Legionella Pneumophila

Project description:Legionella pneumophila (corby) infected blood derived macrophages are infected in a time course experiment (24& 48hours)

2019-01-25 | GSE125559 | GEO

Legionella pneumophila cells after sub-lethal heat shock

Project description:Legionella pneumophila Philadelphia-1 strain was grown to stationary phase in AYE broth and starved in freshwater for 2 hours and RNA was harvested with or without sublethal heat shock via immersion in a 55 degree C hot water bath for 5 minutes

2022-02-15 | GSE196713 | GEO

Legionella pneumophila transcriptome during intracellular multiplication in human macrophages

Project description:Legionella pneumophila is the causative agent of Legionnaires’ disease, an acute pulmonary infection. L. pneumophila is able to infect and multiply in both phagocytic protozoan, such as Acanthamoeba castellanii, and mammalian professional phagocytes. The best-known virulence determinant used by L. pneumophila to infect host cells is a Type IVb translocation system named Icm/Dot, which is used to modify the host cell functions to the benefit of the bacteria. To date the Icm/Dot systeme is known to translocate more than 100 effectors. While the transcriptional response of Legionella to the intracellular environement of A. castelannii as already been investigated, much less is known of how Legionella reacts transcriptionnally inside human macrophages. In this study, the transcriptome of L. pneumophila was monitored during exponential and post-exponential phase in rich AYE broth and during infection of human cultured macrophages by using microarray and a RNA amplification procedure called SCOTS to allow for the study of conditions of low bacterial loads. Among the genes induced intracellularly are those involved in amino acid synthesis pathway leading to L-arginine, L-histidne and L-proline as well as many transport system involved in amino acid and iron uptake. The Icm/Dot systems is not differentially expressed inside cells compare to the E phase control but the effectors are strongly induced. The intracellular transcriptome was further used to identify putative new Icm/Dot effectors and translocation was show to occur for 3 of them. This study provides a comprehensive view of how L. pneumophila react to the human macrophages intracellular environment.

2011-01-01 | GSE23029 | GEO

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