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Comparative analysis of D. shibae wild-type vs. delta-luxI1 supplemented with different acetylated homoserine-lactones

ABSTRACT: Comparison of a quorum sensing null mutant supplemented with C18-HSL, C18en-HSL, C18dien-HSL with D. shibae wild-type in order to identify traits induced by distinct autoinducers in this organism.

ORGANISM(S): Dinoroseobacter shibae DFL 12 = DSM 16493

PROVIDER: GSE42010 | GEO | 2012/11/03

SECONDARY ACCESSION(S): PRJNA178766

REPOSITORIES: GEO

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Comparative analysis of D. shibae wild-type vs. delta-luxI1 supplemented with different acetylated homoserine-lactones

Project description:Comparison of a quorum sensing null mutant supplemented with C18-HSL, C18en-HSL, C18dien-HSL with D. shibae wild-type in order to identify traits induced by distinct autoinducers in this organism. Loop-design, two to three biological replicates of D. shibae wild-type, delta-luxI1 and delta-luxI1 supplemented with 500 nM C18-HSL, C18en-HSL and C18dien-HSL at cultivation start. Samples were taken in mid-exponential growth phase at OD600 0.4

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D. shibae luxI1

Project description:This SuperSeries is composed of the following subset Series: GSE42010: Comparative analysis of D. shibae wild-type vs. delta-luxI1 supplemented with different acetylated homoserine-lactones GSE42011: Cell-density resolved comparative analysis of D. shibae wild-type vs. delta-luxI1 GSE42012: Cell-density resolved comparative analysis of D. shibae wild-type vs. delta-luxI1 overexpressing luxI1 in trans Refer to individual Series

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Cell-density resolved comparative analysis of D. shibae wild-type vs. delta-luxI1 overexpressing luxI1 in trans

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Project description:D. shibae delta-luxI1 overexpressing the luxI1 gene in trans was compared to the wild-type in order to identify quorum sensing controlled traits in this organism. Samples were taken at different optical densities in the exponential phase and in the stationary phase. Samples from Dinoroseobacter shibae DFL 12 wild-type and delta-luxI1pDP1 are compared during exponential (OD600 0.1, 0.2, 0.4, 0.6, 0.8) and stationary phase (6h post maximum OD600), two biological replicates

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Project description:A quorum sensing null mutant was compared with D. shibae wild-type in order to identify quorum sensing controlled traits in this organism. Samples were taken at different optical densities in the exponential phase and in the stationary phase in order to clarify if quorum sensing regulation is dependent on cell-densitiy Samples from Dinoroseobacter shibae DFL 12 wild-type and delta-luxI1 (Dshi_0312) are compared during exponential (OD600 0.1, 0.2, 0.4, 0.6, 0.8) and stationary phase (6h post maximum OD600), two biological replicates

2012-11-03 | E-GEOD-42011 | biostudies-arrayexpress

Integrated transcriptional regulatory network of quorum sensing, replication, and SOS response in Dinoroseobacter shibae

Project description:Transcriptional response of the QS-negative D. shibae strain ∆luxI1 towards externally added 500nM autoinducer (AI) 3-oxo-C14-HSL over a time period of 3 hours.

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Light dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn

Project description:Light dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn. Dinoroseobacter shibae DFL12T (DSM 16493T) and the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn were grown in artificial saltwater minimal medium (SWM) in baffled flasks shaking at 180 rpm and 30 °C and incubation was performed under light, dark and bluelight conditions. D. shibae wild type and mutant strain were grown under aerobic conditions up to the mid exponential growth phase (OD578 nM 0.5).

2023-10-01 | GSE244289 | GEO

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