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MiRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells

ABSTRACT: miRNA expression profiling in tumor-associated BMDCs induced by tumor cells 1D8 or CT-26 in comparison with those cultured in medium.

ORGANISM(S): Human gammaherpesvirus 8 Betapolyomavirus hominis Mus musculus Rattus norvegicus Human alphaherpesvirus 1 JC polyomavirus human gammaherpesvirus 4 Betapolyomavirus macacae Homo sapiens Human immunodeficiency virus 1 Human betaherpesvirus 5 Murid betaherpesvirus 1

PROVIDER: GSE42722 | GEO | 2012/12/05

SECONDARY ACCESSION(S): PRJNA182941

REPOSITORIES: GEO

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miRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells

Project description:miRNA expression profiling in tumor-associated BMDCs induced by tumor cells 1D8 or CT-26 in comparison with those cultured in medium. To prepare tumor-associated BMDCs(Bone Marrow-derived Dendritic Cells), murine BMDCs were co-cultured with 1D8 or CT-26 cells in a transwell plate in 1640 medium supplemented with 3% FCS and 1% penicillin and streptomycin for 24 hrs. Total RNAs were isolated using the TRIZOLM-BM-. Reagent(Invitrogen) according to manufacturerM-bM-^@M-^Ys instructions.The samples were labeled using the miRCURYM-bM-^DM-" Array Power labeling kit (Exiqon). miRNA array hybridization was performed, and unbound miRNA Labels were washed away. Microarrays were analyzed using GenePix 4000B scanner and GenePix Pro 6 software (Molecular Devices).

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