Transcriptomics

Dataset Information

0

A genome-wide functional investigation into the roles of differentially expressed genes and microRNAs, including 24 transcription factor families, in tobacco roots during the drought stress response


ABSTRACT: Drought stress response is a complex trait regulated at multiple levels. In the past few years, molecular and genomic studies have shown that several drought responsive genes (DRGs) with various functions are induced by drought stresses, and that various transcription factors (TFs) are involved in the regulation of stress-inducible genes. In addition to those DRGs mentioned above, microRNAs (miRNAs) are important regulators of gene expression at the posttranscriptional level by repressing mRNA expression. There is a complex interplay between transcriptional and post-transcriptional regulation of drought response that has not been extensively characterized in tobacco. In order to fully understand DRGs (including TFs) and different roles of miRNAs involved in the stress response, we sequenced and analysed three Digital Gene Expression (DGE) libraries in roots from drought treated tobacco plants, and four small RNA populations in roots, stems and leaves from control or drought treated tobacco plants. We identified 276 candidate DRGs in tobacco with sequence similarities to 64 known DRGs from model plants and crops and about 40% were TFs including WRKY, NAC, ERF and bZIP families. Furthermore, Out of these tobacco DRGs, 54differentially expressed DRGs included 21 TFs, which belonged to 24 TFs families such as NAC (6), MYB (4), ERF (10) and bZIP (1). Additionally, we confirmed expression of 39 known miRNA families (122 members) and five conserved miRNA families, which showed differential regulation under drought stress. Targets of miRNAs were further surveyed based on a recently published study, in which ten targets were DRGs. Finally, an integrated gene regulatory network has been proposed for the molecular mechanisms of the response of tobacco roots to drought stress using differentially expressed DRGs, the changed expression profiles of miRNAs and their target transcripts as a basis base on previous studies. In general, our data provide valuable information for future studies of the molecular mechanisms underlying tobacco roots in response to drought resistance in tobacco and other plants.

ORGANISM(S): Nicotiana tabacum

PROVIDER: GSE43058 | GEO | 2013/12/20

SECONDARY ACCESSION(S): PRJNA184200

REPOSITORIES: GEO

Similar Datasets

2013-12-20 | E-GEOD-43058 | biostudies-arrayexpress
2017-11-01 | GSE93081 | GEO
2012-05-09 | GSE29154 | GEO
2012-05-08 | E-GEOD-29154 | biostudies-arrayexpress
2014-11-01 | E-GEOD-33370 | biostudies-arrayexpress
2014-11-01 | GSE33370 | GEO
2016-09-30 | GSE81325 | GEO
2007-09-01 | GSE8161 | GEO
2022-09-26 | GSE214048 | GEO
2021-12-31 | GSE157523 | GEO