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A jasmonate ZIM-domain protein NaJAZd regulates floral jasmonic acid levels and counteracts flower abscission in Nicotiana attenuata plants

ABSTRACT: Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of JAZd after elicitation with oral secretions of Manduca sexta larvae.

ORGANISM(S): Nicotiana attenuata

PROVIDER: GSE43395 | GEO | 2014/12/31

SECONDARY ACCESSION(S): PRJNA185816

REPOSITORIES: GEO

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NaJAZh regulates a subset of defense responses against herbivores and counteracts developmental necrosis in Nicotiana attenuata plants

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of JAZh after elicitation with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata wild type and irJAZh plants was measured at 2 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with irJAZh plants. A total of 480 genes were identified as more the 2 fold differentially regulated in irJAZh plants compared to wild type plants.

2012-07-11 | E-GEOD-33681 | biostudies-arrayexpress

NaJAZh regulates a subset of defense responses against herbivores and counteracts developmental necrosis in Nicotiana attenuata plants

2012-07-12 | GSE33681 | GEO

Characterization of Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 (ir-lecRK1 plants).

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 after elicitation with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata wild type and ir-lecRK1 plants was measured at 1 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with ir-lecRK1 plants. A total of 148 genes were identified as differentially regulated in ir-lecRK1 plants compared to wild type plants.

2011-11-23 | E-GEOD-29905 | biostudies-arrayexpress

Characterization of Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 (ir-lecRK1 plants).

2011-11-23 | GSE29905 | GEO

A jasmonate ZIM-domain protein NaJAZd regulates floral jasmonic acid levels and counteracts flower abscission in Nicotiana attenuata plants.

Project description: Not available

| S-EPMC3585257 | biostudies-literature

NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata

Project description:Identification of significantly altered transcripts in Nicotiana attenuata plants with reduced MYC2 expression after induction with oral secretions of Manduca sexta larvae.

2013-05-17 | GSE45608 | GEO

NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata

Project description:Identification of significantly altered transcripts in Nicotiana attenuata plants with reduced MYC2 expression after induction with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata EV and MYC2_VIGS plants was measured at 1 hour after induction with oral secretions of Manduca sexta larvae. Three independent experiments were performed with EV plants and three independent experiments were performed with MYC2_VIGS plants. A total of 47 genes were identified as differentially regulated in MYC2_VIGS plants compared to EV plants.

2013-05-17 | E-GEOD-45608 | biostudies-arrayexpress

Tissue specific diurnal rhythm of transcripts and their regulation during herbivore attack in Nicotiana attenuata

Project description:Idenfication and characterization of oscillating transcripts after elicitation with oral secretions from the sepcialist herbivore, Manduca sexta larvae

2011-12-29 | GSE30287 | GEO

Tissue specific diurnal rhythm of transcripts and their regulation during herbivore attack in Nicotiana attenuata

Project description:Idenfication and characterization of oscillating transcripts after elicitation with oral secretions from the sepcialist herbivore, Manduca sexta larvae Source leaves, sink leaves and roots were collected every 4 h for one days.

2011-12-29 | E-GEOD-30287 | biostudies-arrayexpress

Herbivore-induced vaccination of N. attenuata and S. nigrum

Project description:Nicotiana attenuata and Solanum nigrum were challenged with different insects: Manduca sexta and Tupiocoris notatus in different combinations Goals of the study are first to haracterize the transcriptional response of two native Solanaceous plants (Nicotiana attenuata and Solanum nigrum) to attack from two herbivorous pests common on Solanaceous crops (Manduca sexta and Tupiocoris notatus). And second to identify genes involved in herbivore vaccination phenomenon. After germination, inbred and genetically characterized lines of N. attenuata DI92 and S. nigrum Sn30 originally collected from southwestern Utah in 1988, and Jena Germany in 2000, respectively, were grown in a greenhouse. Eggs of Manduca sexta (abbreviated M. s.) came from the North Carolina State University - Entomology Insectary and were hatched under 37°C. Nymphs and adults of Tupiocoris notatus (abbreviated T. n.) came from laboratory colonies started in 2000 with individuals collected from our Utah field sites. Plant were challenged with these different insect and combinations of them. All plants for a given treatment were enclosed in glass-and-mesh insect cages to avoid cross-infection. After 24 h or 5 days the herbivores and their frass were removed, and the above ground biomass of each plant was flash-frozen in liquid nitrogen and stored at -80 °C until RNA extraction. Three independent replicate cages (biological replicates) were used for each of the 7 treatments resulting in a total of 21 cages per species. RNA extraction was performed according to the protocol using Trizol. The RNA samples represent a pooled sample from 4 plants grown in a cage. Keywords: Direct comparison

2007-09-01 | GSE8143 | GEO

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