Dataset Information


The function of CEBiP in the chitin elicitor signaling in rice cells

ABSTRACT: To see the function of CEBiP in the chitin elicitor signaling in rice cells, a plasmid for the gene-specific knock-down by RNA interference (RNAi) was constructed by using a sequence for the 3’-terminal region of CEBiP and transformed into the rice cells by using Rhizobium radiobacter (Agrobacterium tumefaciens). chitinoligomer was added (or not added) to the medium. For the control of them, rice cells that non transformed wild type was used. Keywords: genetic modification Overall design: We compared chitinoligomer treated rice cells with non treated on a same plate. Dye-swaps was done for transfomed or non transformed rice cells individually.

INSTRUMENT(S): RICE 22k oligo microarray

SUBMITTER: Chiharu Akimoto-Tomiyama  

PROVIDER: GSE4645 | GEO | 2006-06-13



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Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor.

Kaku Hanae H   Nishizawa Yoko Y   Ishii-Minami Naoko N   Akimoto-Tomiyama Chiharu C   Dohmae Naoshi N   Takio Koji K   Minami Eiichi E   Shibuya Naoto N  

Proceedings of the National Academy of Sciences of the United States of America 20060707 29

Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. To clarify the molecular machinery involved in the perception and transduction of chitin oligosaccharide elicitor, a high-affinity binding protein for this elicito  ...[more]

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