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Genome wide gene-expression analysis of facultative reproductive diapause in the two-spotted spider mite Tetranychus urticae

ABSTRACT: Investigating essential physiological processes in diapausing mites by analyzing genome wide gene expression changes using custom-built microarray. We investigated the molecular biology of facultative reproductive diapause in the chelicerate Tetranychus urticae (Acari: Tetranychidae) by analyzing genome-wide gene expression differences in diapausing and non diapausing T. urticae, using an Agilent custom-built two color gene expression microarray. Analysis of this dataset showed that a remarkable number, 11% of the total number of predicted T. urticae genes, were differentially expressed. Gene Ontology analysis revealed that many metabolic pathways were affected in diapausing females. Genes related to digestion and detoxification, cryo-protection, carotenoid synthesis and the organization of the cytoskeleton were profoundly influenced by the state of diapause. We also further confirmed the importance of horizontally transferred carotenoid synthesis genes in diapause and different color morphs of T. urticae.

ORGANISM(S): Tetranychus urticae

PROVIDER: GSE48858 | GEO | 2013/11/29

SECONDARY ACCESSION(S): PRJNA212014

REPOSITORIES: GEO

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Genome wide gene-expression analysis of facultative reproductive diapause in the two-spotted spider mite Tetranychus urticae

Project description:Investigating essential physiological processes in diapausing mites by analyzing genome wide gene expression changes using custom-built microarray. We investigated the molecular biology of facultative reproductive diapause in the chelicerate Tetranychus urticae (Acari: Tetranychidae) by analyzing genome-wide gene expression differences in diapausing and non diapausing T. urticae, using an Agilent custom-built two color gene expression microarray. Analysis of this dataset showed that a remarkable number, 11% of the total number of predicted T. urticae genes, were differentially expressed. Gene Ontology analysis revealed that many metabolic pathways were affected in diapausing females. Genes related to digestion and detoxification, cryo-protection, carotenoid synthesis and the organization of the cytoskeleton were profoundly influenced by the state of diapause. We also further confirmed the importance of horizontally transferred carotenoid synthesis genes in diapause and different color morphs of T. urticae. We made one comparison: diapausing mites (DIA) vs non-diapausing mites (NON-DIA), in 4 replicates. Both types of mites belonged to the T. urticae LS-VL strain. In this strain, approximately 30% of mites enter diapause under the experimental conditions applied. Hence, we were able to sample RNA of mites with similar genetic background that were reared under identical environmental conditions. The labeled cRNA samples were pooled and hybdrized to a custom Sureprint genome wide G3 Gene Expression 8x60K microarray. Data was normalized by Agilent Feature Extraction software (using protocol GE2_107_SEP09). Genespring software (Agilent technologies) was used for the statistical analysis of the data.

2013-11-29 | E-GEOD-48858 | biostudies-arrayexpress

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2021-04-26 | PXD017150 | Pride

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2012-09-30 | E-GEOD-35622 | biostudies-arrayexpress

Cyflumetofen resistance in the two-spotted spider mite Tetranychus urticae

Project description:Cyflumetofen is a novel acaricide on the international market with an unknown mode of action. Under laboratory conditions, we selected for high levels of cyflumetofen resistance in a susceptible mite strain and performed genome-wide gene-expression analysis. Differential expression between the resistant and susceptible strain was identified to study on the molecular level how mites develop resistance to this novel acaricide. Our results show that in T. urticae, the selection for cyflumetofen resistance resulted in a differential expression in only a limited number of genes. This transcriptomic study provides an unbiased look how cyflumetofen resistance triggers selection on the transcriptional level in T. urticae.

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Transcriptome evaluation of gene expression changes during carotenoid accumulation in the storage root of carrot (Daucus carota)

Project description:In this study, a cross species hybridization (CSH) approach was used to evaluate whole transcriptome changes during carotenoid accumulation in the storage root of carrot (Daucus carota). Carotenoids are isoprenoid compounds providing red, yellow and orange color to plants. Previous gene expression analyses of carotenoid accumulation in non-model plant species have primarily used a candidate gene approach. Since global transcriptome analyses require extensive genome sequence, in the absence of these genomic resources an alternate approach uses platforms developed for model plant species. To assess transcriptome patterns associated with carotenoid pigmentation in carrot storage root, two carrot sibling inbred lines, B8788, true breeding for orange color and B8750, true breeding for white root color, were hybridized to the Medicago Affymetrix GeneChip microarray.

2014-08-13 | GSE49873 | GEO

Cyflumetofen resistance in the two-spotted spider mite Tetranychus urticae

2017-05-12 | E-GEOD-62916 | biostudies-arrayexpress

Cyenopyrafen resistance in the two-spotted spider mite Tetranychus urticae

Project description:Cyenopyrafen is a novel acaricide with a currently unknown mode of action. We selected for high levels of cyenopyrafen resistance in a susceptible spider mite strain and previously published patterns of cross-resistance to other well-described acaricides (Khalighi et al., 2014). In addition, we also included an independent cyenopyrafen-susceptible mite strain (referred to as Akita) in this study. We performed transcriptomic analysis to identify on the molecular level how T. urticae develops resistance to this novel acaricide. Using this dataset, we show that the selection for cyenopyrafen resistance resulted in massive transcriptomic responses in T. urticae. The multi-gene family of cytochrome P450 monooxygenases (CYPs) exhibited a drastic differential expression with a significant transcriptional induction in the acaricide resistant strain. This transcriptomic study highlights the potential importance of CYPs in mite resistance to the cyenopyrafen acaricide.

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Project description:Loliolide, a metabolite of carotenoid metabolic pathways in plants, was identified as an inducer of resistance to herbivores such as the two-spotted spider mite, Tetranychus urticae, and the common cutworm, Spodoptera litura. To identify host factors involved in loliolide-induced herbivore resistance, microarray analysis of tomato plants treated with loliolide was performed. We identified several cell wall-associated defense genes as loliolide-responsive genes.

2018-06-19 | GSE115942 | GEO

proteome analysis adductor muscle color in Boihai Red Scall

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