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Gene expression analysis in macrophages stimulated 18 h with Adiponectin

ABSTRACT: Bone marrow derived macrophages (BMDM) generated from c57bl/6j mice bone marrow cells were stimulated for 18 h with 12 microgram/ml adiponectin, RNA from non-stimulated or 18 h adiponectin-stimulated BMDM subjected to a agilent microarray analysis

ORGANISM(S): Mus musculus

PROVIDER: GSE49332 | GEO | 2014/07/03

SECONDARY ACCESSION(S): PRJNA213640

REPOSITORIES: GEO

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Gene expression analysis in macrophages stimulated 18 h with Adiponectin

Project description:Bone marrow derived macrophages (BMDM) generated from c57bl/6j mice bone marrow cells were stimulated for 18 h with 12 microgram/ml adiponectin, RNA from non-stimulated or 18 h adiponectin-stimulated BMDM subjected to a agilent microarray analysis Three different non-stimulated and 3 different 18 h adiponectin (12 microgram/ml) RNA samples were subjected to a micrrorray analysis

2014-07-03 | E-GEOD-49332 | biostudies-arrayexpress

Expression data from bone marrow cultures treated with adiponectin - evidence for lipopolysaccharide contamination

Project description:The adipocyte-derived hormone adiponectin potently inhibits osteoclast formation in vitro. Gene expression profiling of murine bone marrow cells treated with adiponectin was used to identify candidate genes that mediate the effect of adiponectin to inhibit osteoclastogenesis in these cultures. However, we found that contamination by lipopolysaccharide confounds the study of adiponectin activity. Bone marrow cells were cultured for up to five days and RNA was extratced from cells treated with adiponectin and from untreated controls at different time points.

2012-02-02 | E-GEOD-35466 | biostudies-arrayexpress

Expression data from bone marrow cultures treated with adiponectin - evidence for lipopolysaccharide contamination

2012-02-02 | GSE35466 | GEO

Gene regulation by full length human adiponectin

Project description:Transcriptionnal profiling of 4 human cell lines treated during 3 hours with human adiponectin Keywords: transcriptionnal analysis Four human cell lines (Hela, HepG2, Panc-1, Hek293) were grown until confluence. These cell lines were serum depleted during 2 hours and then treated with human adiponectin (2,5 µg/ml) during 3 hours. Each adiponectin stimulated cell line was compared to depleted cell line. Depleted cell line was considered as control.

2010-12-09 | E-GEOD-14070 | biostudies-arrayexpress

High-throughput RNA Sequencing of BMDM of Mus musculus stimulated by C. auris or C. albicans

Project description:Candida auris has been globally recognized as a multidrug-resistant human fungal pathogen that contributes for the worldwide occurrence of nosocomial outbreaks. It has been reported that C. auris was able to avoid neutrophil attack, suggestive of an impaired innate immune response. Whether C. auris evades the innate immune recognition of BMDM (bone marrow derived macrophage) remains poorly understood, and as for well-known Candida species -C. albicans, it can trigger immune response. To determine whether occurs difference between immune response stimulated by C. auris or C. albicans, we performed mRNA-seq of BMDM stimulated by C. auris or C. albicans.

2022-05-21 | GSE203508 | GEO

Differential gene expression stimulated by acGM in bone marrow derived macrophages

Project description:To explore the mechanism of inflammation caused by glucomannan with increasing degree of acetyl substitution (acGM) in bone marrow derived macrophages (BMDM), BMDM was treated with acGM-0.2 and acGM-1.8 for 24h.

2019-06-10 | GSE129591 | GEO

RNA-seq Analysis of BMDM treated with cancer-cell conditioned medium in the presence or abscence of lipid contents

Project description:mRNA profiles of bone-marrow derived macrophages (BMDM) stimulated by cancer cell-conditioned medium in the presence or absnce of lipid contents

2021-06-14 | GSE166735 | GEO

Proteomic analysis of GM-CSF and M-CSF polarized bone marrow derived macrophages

Project description:Bone marrow cells were isolated, primed with M-CSF (M-BMDM) or GM-CSF (GM-BMDM) and cultured for 7 days. The proteomic difference between GM-BMDM and M-BMDM were analyzed to describe the phenotye and function of two types of macrophages.

2023-03-29 | PXD041180 | iProX

Predicting gene level sensitivity to JAK-STAT signaling perturbation using a mechanistic-to-machine learning framework I

Project description:To link upstream IL-6 and IL-10-induced JAK-STAT signaling to downstream gene expression in bone marrow derived macrophages (BMDMs). Bulk RNAseq was done on cytokine-stimulated BMDM in order to profile cytokine-induced response.

2023-08-07 | GSE231343 | GEO

Sequencing of LPS-stimulated immune cells from chicken

Project description:RNAs were analysed from bone marrow derived dendritic cells from 6 week old birds (control and LPS stimulated), bone marrow derived macrophages from 6 week old birds (control and LPS stimulated) and heterophils isolated from blood of day-old chicks (control and LPS stimulated).

2015-01-19 | E-MTAB-2996 | biostudies-arrayexpress

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