Project description:Effects of aldosterone on the transcriptome in distal colon. Expression of genes was studied in distal colon surface cells from aldosterone treated vs. vehicle treated rats.
Project description:Kidney distal convoluted tubules (DCT) are important for regulation of urinary salt excretion. Aldosterone is known to exert long-term effects in this segment, and regulate sodium reabsorption via increasing abundance of the Na+-Cl- cotransporter (NCC) and the epithelial Na channel ENaC. Whether acute effects of aldosterone occur in the DCT and the potential signaling networks remain unknown. Here in this study, we aim to identify the acute aldosterone-mediated signaling (rapid effects) in the DCT.
Project description:Aldosterone excess is involved in cardiac diseases. The mechanisms are still unclears. Mice were treated for 7 days with aldosterone and a whole genome microarray analysis was performed.
Project description:Aldosterone excess is involved in cardiac diseases. The mechanisms are still unclears. Mice were treated for 7 days with aldosterone and a whole genome microarray analysis was performed. Two-group experiment: Untreated mice (Ctrl), mice + aldosterone treatment (Aldo). One comparisons: Ctrl vs Aldo
Project description:Compositional analysis of the neonatal rats distal colon mucus proteome by label-free mass spectrometry indicated significant variability in mucus protein composition at different ages.
Project description:The mineralocorticoid receptor (MR, Nr3c2) is responsible for aldosterone-regulation of Na+ and K+ balance and blood pressure. We apply RNA-Seq and bioinformatic approaches in isolated tubule segments of MR KO vs. Control mice to understand how aldosterone activates electrogenic Na+- K+ exchange in the aldosterone sensitive distal nephron (ASDN), including connecting tubule and cortical collecting duct tubule. MR-flox/Pax8‐rtTA/LC1 mice were used as a doxycycline (DOX)-inducible Nr3c2 gene KO model. After DOX treatment, four groups were prepared to distinguish between K+ and MR effects: 1) control mice on normal K+ diet (CT-NK) or 2) high K+ diet (CT-HK) and 3) MR knockout mice on normal K+ diet (KO-NK) or 4) low K+ diet (KO-LK). RNA-Seq analysis was carried out in the microdissected connecting tubule and cortical collecting duct tubule segments (5-6 mice per group and ~10 fresh ASDN tubules per mouse). Differential expression (DE) genes were identified (FDR< 0.05) and used for further bioinformatic analyses. DE genes were identified from comparisons of MR KO-NK vs. CT-NK and MR KO-LK vs. CT-HK, respectively. Absence of transcripts on the third exon of Nr3c2 gene confirmed complete disruption of Nr3c2 gene in the MR KO. All known aldosterone-response genes were significantly decreased in MR KO-LK compared to CT-HK. Our data provide a comprehensive MR-dependent transcriptomic profile of the ASDN isolated from the kidney.
Project description:Aldosterone is arguably the single most important hormone implicated in the control of blood pressure and extracellular fluid volume in mammals. It acts primarily by increasing the rate of transepithelial sodium transport in the kidney tubules. Several monogenetic defects resulting in hypertension have now been attributed to abnormalities in sodium handling within the aldosterone-sensitive distal nephron, where the epithelial sodium channel, ENaC, constitutes the rate-limiting step of sodium transport. To date, the transcription-dependent aldosterone-signaling pathway between receptor and membrane transport effectors, remains incompletely understood. The broad aim of our study is to explore these intracellular signaling pathways that are critical to the functioning of sodium channels. Microarray analysis in an aldosterone-responsive kidney cortical collecting duct cell line (mpkCCDc14), allowed us to identify many potential aldosterone-regulated transcripts. The present study describes the identification, and possible physiological relevance of various aldosterone-regulated transcripts. The results of this study promise to extend our understanding of the molecular basis of aldosterone-regulated sodium transport in kidney epithelia, and provide approaches for regulating this process in disease states such as congestive heart failure and hypertension. Keywords: hormone effect
Project description:The steroid hormone aldosterone plays a role in vascular function and disease. Aldosterone activates the mineralocorticoid receptor (MR), a ligand-activated transcription factor. MR have been found to be expressed in vascular cells and vessels. We used microarrays to identify the global programme of gene expression changes in mouse aortas treated with vehicle (DMSO) or aldosterone.