Project description:Old C57BL/6 mice cannot mount an effective innate immune response We used mouse microarrays to detail the molecular profile of the events that occur following infection
Project description:Innate immunity is fundamental to recognition and clearance of bacterial infection. The relevant cells and molecules that orchestrate an effective response, however, remain incompletely understood. Here we describe a previously unknown population of B cells, which we have named innate response activator (IRA) B cells that recognize bacteria directly through TLR-4-MyD88 and protect against polymicrobial sepsis. IRA-B cells have a unique IgM high CD23 low CD43 + CD93+ GM-CSF+ signature, develop and diverge from B1a B cells, require BAFFR, and adhere to tissue via VLA-4 and LFA-1. B cell subsets are sorted from the spleen and peritoneum of C57BL/6 mice that were given intraperitoneal injections of LPS once daily for four days.
Project description:Innate immunity is fundamental to recognition and clearance of bacterial infection. The relevant cells and molecules that orchestrate an effective response, however, remain incompletely understood. Here we describe a previously unknown population of B cells, which we have named innate response activator (IRA) B cells that recognize bacteria directly through TLR-4-MyD88 and protect against polymicrobial sepsis. IRA-B cells have a unique IgM high CD23 low CD43 + CD93+ GM-CSF+ signature, develop and diverge from B1a B cells, require BAFFR, and adhere to tissue via VLA-4 and LFA-1.
Project description:This study focuses on inflammatory bowel disease gene expression profiling. Surgical specimens from 134 patients undergoing bowel resection for inflammatory bowel disease (IBD) and non IBD controls at Mount Sinai Medical Center were collected as the source of tissue. Control samples (CLs) were harvested from normal non inflamed bowel located more than 10 cm away from the tumor from patients undergoing bowel resection for sporadic colon cancer. Ulcerative colitis (UC) and Crohn’s (CD) patient samples were all isolated from areas containing moderate to severe inflammation. The diagnostic pathology report for each specimen was provided by the Mount Sinai Hospital Pathology Department. Patients with UC and patients with CD shared common medications including corticosteroids, infliximab, azathioprine, and mesalamine.
Project description:Analysis of plasma proteomes from 8 week old C57BL/6J WT or TCRα-/- (Tcratm1Phi) either naïve or infected by intravenous injection of 5x105 yeast (clinical isolate SC5314), and collected 72 hrs post-infection