Project description:Gene expression profiling of normal cervix, CIN's and cancer cervix, with an intent to identify genes involved in the malignant transformation of normal cervix and to identify genes which can be used as biomarkers for early diagnosis. Identification of genes for predicting radiation response using microarray gene expression studies Keywords: Patient tissue samples
Project description:mRNA, lncRNA, and miRNA signatures were identified to discriminate cervical adenocarcinoma from normal cervix by whole transcriptome sequencing. We confirmed the RNA-seq data in another cohort of clinical cervical tissue samples by qRT-PCR.We demonstrated that miR-192-5p/HNF1A-AS1/VIL1 panel could accurately discriminates adenocarcinoma from normal cervix. Moreover,we also identified the circRNA expression profiles in cervical adenocarcinoma tissues and explored the function roles and mechanisms of circular RNA circEYA1 and circSPIDR in cervical adenocarcinoma cells.
Project description:Transcription profiling of cancerous and non cancerous lung adenocarcinoma tissue. Tumour and normal samples from human lung carcinoma from 18 patients plus tumour only from 5 patients
Project description:This SuperSeries is composed of the following subset Series: GSE30758: Epigenome analysis of normal cells from the uterine cervix in a nested prospective case control study within the ARTISTIC trial GSE30759: Epigenome analysis of normal and cancer tissue from the uterine cervix Refer to individual Series
Project description:Gene expression profiling of normal cervix, CIN's and cancer cervix, with an intent to identify genes involved in the malignant transformation of normal cervix and to identify genes which can be used as biomarkers for early diagnosis. Identification of genes for predicting radiation response using microarray gene expression studies Keywords: Patient tissue samples Two dye experiments using Universal control RNA (Stratagene) and RNA from tissues. Biological replicates: Normal = 5; CIN1=4; CIN3=3; Cervical cancers = 28. One replicate per array.
Project description:10 normal squamous cervical epitheilia samples, 7 high grade squamous intraepithelial lesions, and 21 invasive squamous cell carcinomas of the cervix samples were obtained using laser capture miicrodissection. Two rounds of T7-based linear RNA amplification using the Arcturus RiboAmp kit were performed for each sample, and assayed using Affymetrix HG_U133A arrays. Keywords: disease state analysis
Project description:CircRNAs have been found to regulate mRNA expression levels and serve an important role in cervix carcinogenesis. To explore the circRNA expression profiles during the development and progression of cervical cancer, we performed microarray analysis with total RNA in normal cervical epithelium(n=7), HPV16 positive high-grade squamous intraepithelial lesion (HSIL)(n=6), and HPV16 positive cervical squamous cell carcinoma tissues(n=7).