Project description:expression profile of TC-1 tumor 14 days after s.c. samples are mixture of 3 tumors respectively TC-1 tumor from WT_vs_TC-1 tumor from fat-1
Project description:RNA sequencing data of TPC-1 cells (TC cell line) after co-culture with TC-induced macrophages. We used an in vitro co-culture model of a human TC cell line, TPC1 (RET/PTC rearrangement), and human primary monocytes.
Project description:Ewing's Sarcoma cell lines were made resistant to different IGF-1R drugs to investigate mechanisms and pathways modulated by the resistance. EWS TC-71 cell line was exposed to increasing concentration to three different anti-IGF-1R drugs (HAb AVE1642, TKI NVP-AEW541, HAb CP-751,871, cell lines named respectively as TC/AVE, TC/AEW or TC/CP) for at least six months. Expression profile of resistant cell variants was compared either singularly for each resistance or commonly vs. parental cell line. Two technical replicates for resistant variants and three biological replicated for parental cell were present.
Project description:We performed ChIPseq on histone modification marks, transcriptional factors and chromatin architectural proteins in TC-32 and TC-71 Ewing sarcoma cell lines.
Project description:We found that LSD1 inhibition by a monoamine oxidase inhibitor, tranylcypromine (TC), could enhance fetal gamma globin expression. Global effects of TC on erythroid expession were conducted by HG-U219 array strips. Primary human erythroid cells, which differentiated from CD34+ cells for 8 days, were harvested before or and after TC treatment (0.5 µM, 1.5 µM or 5 µM) for the gene expression analysis.
Project description:Cytokine and chemokine expression profiles were generated to characterize treatment- and radioresistance-associated differences in the tumor microenvironment of a TC-1 mouse model. Using parental TC-1 and radiation-resistant TC-1/IR tumors established in syngeneic mice, cytokine protein profiling was performed on tumor lysates following different treatment conditions, including radiotherapy, IL-7–based immune modulation, and their combination. This dataset enables comparative analysis of intratumoral cytokine landscapes across resistance status and treatment modalities in an in vivo setting.