Transcriptomics,Genomics

Dataset Information

37

Effects of Changes in Food Supply at the Time of Sex Differentiation on the Gonadal Transcriptome of Juvenile Fish. Implications for Natural and Farmed Populations


ABSTRACT: Background: Food supply is a major factor influencing growth rates in animals. This has important implications for both natural and farmed fish populations, since food restriction may difficult reproduction. However, a study on the effects of food supply on the development of juvenile gonads has never been transcriptionally described in fish. Methods and Findings: This study investigated the consequences of growth on gonadal transcriptome of European sea bass in: 1) 4-month-old sexually undifferentiated fish, comparing the gonads of fish with the highest vs. the lowest growth, to explore a possible link between transcriptome and future sex, and 2) testis from 11-month-old juveniles where growth had been manipulated through changes in food supply. The four groups used were: i) sustained fast growth, ii) sustained slow growth, iii) accelerated growth, iv) decelerated growth. The transcriptome of undifferentiated gonads was not drastically affected by initial natural differences in growth. Further, changes in the expression of genes associated with protein turnover were seen, favoring catabolism in slow-growing fish and anabolism in fast-growing fish. Moreover, while fast-growing fish took energy from glucose, as deduced from the pathways affected and the analysis of protein-protein interactions examined, in slow-growing fish lipid metabolism and gluconeogenesis was favored. Interestingly, the highest transcriptomic differences were found when forcing initially fast-growing fish to decelerate their growth, while accelerating growth of initially slow-growing fish resulted in full transcriptomic convergence with sustained fast-growing fish. Conclusions: Food availability during sex differentiation shapes the juvenile testis transcriptome, as evidenced by adaptations to different energy balances. Remarkably, this occurs in absence of major histological changes in the testis. Thus, fish are able to recover transcriptionally their testes if they are provided with enough food supply during sex differentiation; however, an initial fast growth does not represent any advantage in terms of transcriptional fitness if later food becomes scarce. Overall design: Whole gonad transcriptome analysis from 35 individuals. Two sampling points at 133 days post fertilization (dpf) (7 individuals from the Fast growing group F and 8 from the Slow growing one S and at 332 dpf (5 individuals from each of the following groups: Fast growing-Fast growing FF; Fast growing-Slow growing FS; Slow growing-Fast growing SF and Slow growing-Slow growing SS).

INSTRUMENT(S): Agilent-024502 Dicentrarchus labrax oligonucleotide-microarray (LOQ) (Probe Name version)

SUBMITTER: Noelia T Díaz Blanco  

PROVIDER: GSE54362 | GEO | 2014-12-25

SECONDARY ACCESSION(S): PRJNA236318

REPOSITORIES: GEO

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Publications

Effects of changes in food supply at the time of sex differentiation on the gonadal transcriptome of juvenile fish. Implications for natural and farmed populations.

Díaz Noelia N   Ribas Laia L   Piferrer Francesc F  

PloS one 20141023 10


Food supply is a major factor influencing growth rates in animals. This has important implications for both natural and farmed fish populations, since food restriction may difficult reproduction. However, a study on the effects of food supply on the development of juvenile gonads has never been transcriptionally described in fish.This study investigated the consequences of growth on gonadal transcriptome of European sea bass in: 1) 4-month-old sexually undifferentiated fish, comparing the gonads  ...[more]

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