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Alterations in the transcriptome of soybean in response to enhanced somatic embryogenesis promoted by 35S:GmAGL15

ABSTRACT: A soybean ortholog of the Arabidopsis MADS-domain transcription factor (called GmAGL15) enhanced somatic embryogenesis from immature cotyledon explants of soybean when expressed via the 35S promoter compared to non transgenic tissue (cultivar Jack). To better understand how this occurs an expression microarray experiment was performed. publication: Q. Zheng and S.E. Perry. (2014). Alterations in the transcriptome of soybean in response to enhance somatic embryogenesis promoted by orthologs of AGAMOUS-Like 15 and AGAMOUS-Like 18. Plant Physiology, in press.

ORGANISM(S): Glycine max

PROVIDER: GSE54523 | GEO | 2014/01/30

SECONDARY ACCESSION(S): PRJNA236768

REPOSITORIES: GEO

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Alterations in the transcriptome of soybean in response to enhanced somatic embryogenesis promoted by 35S:GmAGL15

Project description:A soybean ortholog of the Arabidopsis MADS-domain transcription factor (called GmAGL15) enhanced somatic embryogenesis from immature cotyledon explants of soybean when expressed via the 35S promoter compared to non transgenic tissue (cultivar Jack). To better understand how this occurs an expression microarray experiment was performed. publication: Q. Zheng and S.E. Perry. (2014). Alterations in the transcriptome of soybean in response to enhance somatic embryogenesis promoted by orthologs of AGAMOUS-Like 15 and AGAMOUS-Like 18. Plant Physiology, in press. Comparison of transcriptomes of non-transgenic tissue to 35S:GmAGL15 tissue at the time of explant preparation (0 days) and 3 and 7 days after induction of somatic embryogenesis by placement on medium containing 2,4-D.

2014-01-30 | E-GEOD-54523 | biostudies-arrayexpress

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Gene expression in response to AGL15 during somatic embryogenesis

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Gene expression in response to AGL15 during somatic embryogenesis

Project description:Transcript accumulation was measured using the Affymetrix Arabidopsis ATH1 Genome Array [ATH1-121501] to document changes in response to the MADS-domain transcription factor AGAMOUS-Like 15 during somatic embryogenesis. A somatic embryo system was used where mature seed is allowed to complete germination in liquid MS media containing 2,4-D and seedlings produce somatic embryos from the shoot apical meristem (SAM) region. The frequency with which these embryos are produced directly correlates with AGL15 accumulation. Experiment Overall Design: Wild type Arabidopsis (Columbia ecotype) with normal amounts of AGL15 were compared to (1) 35S:AGL15 that constitutively expresses AGL15 and produces more somatic embryos from the shoot apical region than wild type and (2) agl15 agl18 double loss-of-function mutants that produce less somatic embryos from the shoot apical region than wild type.

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Arabidopsis thaliana

Project description:embryogenesis ws-somatic embryogenesis

| PRJNA143431 | ENA

Arabidopsis thaliana

Project description:embryogenesis col0-somatic embryogenesis

| PRJNA143393 | ENA

Project description:somatic embryogenesis

| PRJNA808630 | ENA