Dataset Information


Transcriptional profiling of a Staphylococcus aureus clinical isolate UAMS1 and its isogenic agr and sarA mutants

ABSTRACT: The purpose of this study was to compare the global, growth phase-dependent transcriptional profiles of two isolates of Staphylococcus aureus. One isolate is a prototypic laboratory strain named RN6390, and has been used frequently as a model organism for study of staphylococcal physiology and virulence. However, recent studies indicate that RN6390 is not, in general, genotypically or phenotypically representative of clinical isolates of Staphyloccos aureus. Therefore, there is no current comprehensive picture of gene expression patterns in a virulent, clinical isolate of Staphyloccous aureus. For these reasons, we compare the transcriptional profile of RN6390 to that of a virulent clinical isolate, UAMS-1. Also included in this study is profiling of two UAMS-1 regulatory mutants, UAMS-155, and UAMS-929. These strains possess mutations in the accessory gene regulator (agr) and staphylococcal accessory regulator (sarA) genes, respectively. These two genes are well described global regulatory molecules that are reported to play important roles in controlling virulence factor production and biofilm formation in Staphylococcus aureus. However, most study of these two molecules has been limited to laboratory strains such as RN6390. For these reasons, this study also includes transcriptional profiling of UAMS agr and sarA mutants. Keywords: Comparative, growth phase-dependent transcriptional profiling of bacterial strains and isogenic regulatory mutants Overall design: For the experiments comparing UAMS-1 to RN6390, two replicate hybridizations were performed using two independently isolated RNA samples were performed for each growth phase and each strain. Likewise, for both the agr and sarA profiling experiments, two replicate hybridizations were performed using two independently-isolated RNA samples were performed for each growth phase and each mutant.

INSTRUMENT(S): Saur2a custom-made Affymetrix array

SUBMITTER: Ellen Murphy  

PROVIDER: GSE5466 | GEO | 2006-10-03



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Transcriptional profiling of a Staphylococcus aureus clinical isolate and its isogenic agr and sarA mutants reveals global differences in comparison to the laboratory strain RN6390.

Cassat James J   Dunman Paul M PM   Murphy Ellen E   Projan Steven J SJ   Beenken Karen E KE   Palm Katherine J KJ   Yang Soo-Jin SJ   Rice Kelly C KC   Bayles Kenneth W KW   Smeltzer Mark S MS  

Microbiology (Reading, England) 20061001 Pt 10

The production of Staphylococcus aureus virulence factors is under the control of complex regulatory circuits. Most studies aimed at defining these regulatory networks have focused on derivatives of the strain NCTC 8325, most notably RN6390. However, all NCTC 8325 derivatives, including RN6390, possess an 11 bp deletion in rsbU. This deletion renders NCTC 8325 derivatives naturally sigma-factor-B deficient. Recent studies have shown that RN6390 is also deficient, in comparison to clinical isolat  ...[more]

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