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Targets of AtWRKY27 in Arabidopsis leaves

ABSTRACT: The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. Experimenter name = Shahid Mukhtar Experimenter phone = +49-221-5062-310 Experimenter fax = +49-221-5062-353 Experimenter address = Max.Planck-Institute for Plant Breeding Experimenter address = Dept. Plant Microbe Interactions Experimenter address = Carl-von-Linne Weg 10 Experimenter address = Koeln Experimenter zip/postal_code = 50829 Experimenter country = Germany Keywords: genetic_modification_design

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE5537 | GEO | 2006/08/15

SECONDARY ACCESSION(S): PRJNA96023

REPOSITORIES: GEO

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Transcription profiling of Arabidopsis leaves to identify targets of AtWRKY27 in

Project description:The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. Experimenter name = Shahid Mukhtar; Experimenter phone = +49-221-5062-310; Experimenter fax = +49-221-5062-353; Experimenter address = Max.Planck-Institute for Plant Breeding; Experimenter address = Dept. Plant Microbe Interactions; Experimenter address = Carl-von-Linne Weg 10; Experimenter address = Koeln; Experimenter zip/postal_code = 50829; Experimenter country = Germany Experiment Overall Design: 4 samples were used in this experiment

2008-06-13 | E-GEOD-5537 | biostudies-arrayexpress

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2008-06-15 | E-GEOD-6829 | biostudies-arrayexpress

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