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Group II-A WRKY transcription factors and early leaf senescence

ABSTRACT: In our laboratory we are interested in studying the functions of WRKY zinc finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. trichome development and plant leaf senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors in response to bacterial infection. Our aim is to compare and contrast the gene expression profiles of 3-week-old wild type and WRKY T-DNA knockout mutants grown in a growth chamber under long day growth conditions and subsequently challenged for 6 hours with the virulent bacterial pathogen /Pseudomonas syringae/ DC3000. Experimenter name: Bekir Uelker Experimenter phone: 49-221-5062-310 Experimenter fax: 49-221-5062-353 Experimenter department: Somsissich Lab Experimenter institute: Max-Planck-Institute for Plant Breeding Research Experimenter address: Department of Plant Microbe Interactions Experimenter address: Carl-von-Linne-Weg 10 Experimenter address: Cologne Experimenter zip/postal_code: 50829 Experimenter country: Germany Keywords: genetic_modification_design

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE6829 | GEO | 2007/01/22

SECONDARY ACCESSION(S): PRJNA99153

REPOSITORIES: GEO

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Group II-A WRKY transcription factors and early leaf senescence (2)

Project description:In our laboratory we are interested in studying the functions of WRKY zink finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. plant senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors. Our aim to compare and contrast the gene expression profiles of 35 days-old untreated wild type and WRKY T-DNA knockout plants grown in a growth chamber under long day growth conditions. All plants chosen at this stage showed slight yellowing of the first two to four leaves. Experimenter name: Bekir Uelker Experimenter phone: 49-221-5062-310 Experimenter fax: 49-221-5062-353 Experimenter department: Somsissich Lab Experimenter institute: Max-Planck-Institute for Plant Breeding Research Experimenter address: Department of Plant Microbe Interactions Experimenter address: Carl-von-Linne-Weg 10 Experimenter address: Cologne Experimenter zip/postal_code: 50829 Experimenter country: Germany Keywords: genetic_modification_design

2007-01-22 | GSE6830 | GEO

Transcription profiling of Arabidopsis Group II-A WRKY transcription factor knockouts - early leaf senescence (2)

Project description:In our laboratory we are interested in studying the functions of WRKY zink finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. plant senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors. Our aim to compare and contrast the gene expression profiles of 35 days-old untreated wild type and WRKY T-DNA knockout plants grown in a growth chamber under long day growth conditions. All plants chosen at this stage showed slight yellowing of the first two to four leaves. Experimenter name: Bekir Uelker; Experimenter phone: 49-221-5062-310; Experimenter fax: 49-221-5062-353; Experimenter department: Somsissich Lab; Experimenter institute: Max-Planck-Institute for Plant Breeding Research; Experimenter address: Department of Plant Microbe Interactions; Experimenter address: Carl-von-Linne-Weg 10; Experimenter address: Cologne; Experimenter zip/postal_code: 50829; Experimenter country: Germany Experiment Overall Design: 8 samples were used in this experiment

2008-06-15 | E-GEOD-6830 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and WRKY T-DNA knockout mutants challenged with Pseudomonas syringae

Project description:In our laboratory we are interested in studying the functions of WRKY zinc finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. trichome development and plant leaf senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors in response to bacterial infection. Our aim is to compare and contrast the gene expression profiles of 3-week-old wild type and WRKY T-DNA knockout mutants grown in a growth chamber under long day growth conditions and subsequently challenged for 6 hours with the virulent bacterial pathogen /Pseudomonas syringae/ DC3000. Experimenter name: Bekir Uelker; Experimenter phone: 49-221-5062-310; Experimenter fax: 49-221-5062-353; Experimenter department: Somsissich Lab; Experimenter institute: Max-Planck-Institute for Plant Breeding Research; Experimenter address: Department of Plant Microbe Interactions; Experimenter address: Carl-von-Linne-Weg 10; Experimenter address: Cologne; Experimenter zip/postal_code: 50829; Experimenter country: Germany Experiment Overall Design: 7 samples were used in this experiment

2008-06-15 | E-GEOD-6829 | biostudies-arrayexpress

Targets of AtWRKY27 in Arabidopsis leaves

Project description:The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. Experimenter name = Shahid Mukhtar Experimenter phone = +49-221-5062-310 Experimenter fax = +49-221-5062-353 Experimenter address = Max.Planck-Institute for Plant Breeding Experimenter address = Dept. Plant Microbe Interactions Experimenter address = Carl-von-Linne Weg 10 Experimenter address = Koeln Experimenter zip/postal_code = 50829 Experimenter country = Germany Keywords: genetic_modification_design

2006-08-15 | GSE5537 | GEO

Transcription profiling of Arabidopsis leaves to identify targets of AtWRKY27 in

Project description:The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. Experimenter name = Shahid Mukhtar; Experimenter phone = +49-221-5062-310; Experimenter fax = +49-221-5062-353; Experimenter address = Max.Planck-Institute for Plant Breeding; Experimenter address = Dept. Plant Microbe Interactions; Experimenter address = Carl-von-Linne Weg 10; Experimenter address = Koeln; Experimenter zip/postal_code = 50829; Experimenter country = Germany Experiment Overall Design: 4 samples were used in this experiment

2008-06-13 | E-GEOD-5537 | biostudies-arrayexpress

FRI FLC combos

Project description:This experiment was annotated by TAIR (http://arabidopsis.org).FRI FLC time series Experimenter name = Markus Schmid Experimenter phone = ++49-7071-601-1413 Experimenter fax = ++49-7071-601-1412 Experimenter department = Detlef Weigel Laboratory Germany Experimenter institute = Max-Planck-Institute for Developmental Biology Experimenter address = Dept. of Molecular Biology Experimenter address = Spemannstr. 37-39 Experimenter address = Tübingen Experimenter zip/postal_code = 72076 Experimenter country = Germnay Keywords: time_series_design; strain_or_line_design; innate_behavior_design

2007-01-08 | GSE5746 | GEO

Transcription profiling of Arabidopsis flc3/fri mutants under different light conditions

Project description:This experiment was annotated by TAIR (http://arabidopsis.org).FRI FLC time series; Experimenter name = Markus Schmid; Experimenter phone = ++49-7071-601-1413; Experimenter fax = ++49-7071-601-1412; Experimenter department = Detlef Weigel Laboratory Germany; Experimenter institute = Max-Planck-Institute for Developmental Biology; Experimenter address = Dept. of Molecular Biology; Experimenter address = Spemannstr. 37-39; Experimenter address = TÃ¼bingen; Experimenter zip/postal_code = 72076; Experimenter country = Germnay Experiment Overall Design: 36 samples were used in this experiment

2008-06-14 | E-GEOD-5746 | biostudies-arrayexpress

Response to CBF2 expression

Project description:This experiment was annotated by TAIR (http://arabidopsis.org). This experiment looks at changes in gene expression in response to constitutive expression of the transcription factor CBF2. Experimenter name = Jonathan Vogel Experimenter phone = 517-355-2299 Experimenter fax = 517-353-5174 Experimenter department = MSU-DOE Plant Research Lab Experimenter institute = Michigan State University Experimenter address = East Lansing Experimenter zip/postal_code = MI 48824 Experimenter country = USA Keywords: genetic_modification_design

2006-08-15 | GSE5536 | GEO

Response to ZAT12 expression

Project description:This experiment was annotated by TAIR (http://arabidopsis.org). This experiment looks at changes in gene expression in response to constitutive expression of the transcription factor ZAT12. Experimenter name = Jonathan Vogel Experimenter phone = 517-355-2299 Experimenter fax = 517-353-5174 Experimenter department = MSU-DOE Plant Research Lab Experimenter institute = Michigan State University Experimenter address = East Lansing Experimenter zip/postal_code = MI 48824 Experimenter country = USA Keywords: genetic_modification_design

2007-01-08 | GSE5742 | GEO

Response to cold, soil grown plants

Project description:This experiment was annotated by TAIR (http://arabidopsis.org). This experiment looks at changes in gene expression in Col-0 plants grown in soil in response to low temperature over time. Experimenter name = Jonathan Vogel Experimenter phone = 517-355-2299 Experimenter fax = 517-353-5174 Experimenter department = MSU-DOE Plant Research Lab Experimenter institute = Michigan State University Experimenter address = East Lansing Experimenter zip/postal_code = MI 48824 Experimenter country = USA Keywords: time_series_design

2006-08-15 | GSE5535 | GEO

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