Genomics

Dataset Information

38

Expression data from Normal (control) and Diabetic animals LV heart tissue (At 16wks)


ABSTRACT: Analysis of gene expression changes in the LV of a rodent heart that occur with uncontrolled diabetes We used microarrays to detail the global changes that occur in gene expression in the LV of the heart as a result of STZ-induced diabetes. Keywords: single time point, comparison control animal v. diabetic animal Overall design: Male wistars were anaesthetised (Halothane 2-5%) and randomly received a tail vein injection of either saline or STZ 60mg/kg. Animals were recovered and housed in pairs on fibrecycle bedding (12 hr light:dark cycle, 50-70 % humidity, 19-21 °C) for the duration of the study. Animal weight and blood glucose were recorded weekly. After 16wks the whole heart was excised from the animal after cervical dislocation and perfused with 60ml of DEPC treated PBS pH 7.4 at 15ml/min. LV was dissected away and stored in RNA later (Qiagen) at -80C.RNA from 7 animals from each of the two groups was hybridised to an Affymetrix Rat Genome 230 2.0 Genechip and the resulting changes in gene expression were analysed.

INSTRUMENT(S): [Rat230_2] Affymetrix Rat Genome 230 2.0 Array

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Sarah Glyn-Jones  

PROVIDER: GSE5606 | GEO | 2006-10-31

SECONDARY ACCESSION(S): PRJNA96895

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE5606_RAW.tar Raw
filelist.txt Txt
Items per page:
1 - 2 of 2

Similar Datasets

2008-06-13 | E-GEOD-5606 | ArrayExpress
2008-01-01 | S-EPMC2439860 | BioStudies
1000-01-01 | S-EPMC5702519 | BioStudies
1000-01-01 | S-EPMC4153843 | BioStudies
2020-01-01 | S-EPMC7046677 | BioStudies
2018-01-01 | S-EPMC5857913 | BioStudies
| PRJNA419123 | ENA
2019-01-01 | S-EPMC6812823 | BioStudies
2016-01-01 | S-EPMC5067236 | BioStudies
2011-06-24 | E-GEOD-30194 | ArrayExpress