Transcriptomics

Dataset Information

35

MicroRNA-15/16 Antagonizes c-Myb to Control Natural Killer Cell Maturation (c-Myb overexpression)


ABSTRACT: NK cells develop in the bone marrow and complete their maturation in peripheral organs, but the molecular events controlling maturation are incompletely understood. Utilizing an NK cell-specific miR-15/16 deficient genetic model (15aKO), we identified a critical role for miR-15/16 family microRNAs in the normal maturation of NK cells in vivo, with a specific reduction in mature CD11b+CD27- NK cells in multiple tissues. The mechanism responsible was a block in differentiation, since accelerated NK cell death was not evident, and earlier intermediates of NK cell maturation were expanded. Further, we identified Myb as a direct target of miR-15/16 in NK cells, with Myb expression increased in immature 15aKO NK cells. Following adoptive transfer, immature 15aKO NK cells exhibited defective maturation, which was rescued by ectopic miR-15/16 expression or Myb knockdown. Moreover, Myb overexpression resulted in defective NK cell maturation. Thus, miR-15/16 regulation of Myb controls the normal NK cell maturation program. Overall design: KY-1 and KY-2 cell lines overexpressing c-Myb

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

ORGANISM(S): Mus musculus  

SUBMITTER: Todd A Fehniger  

PROVIDER: GSE58268 | GEO |

SECONDARY ACCESSION(S): PRJNA251855

REPOSITORIES: GEO

Similar Datasets

2015-09-03 | E-GEOD-58268 | ArrayExpress
2015-09-03 | E-GEOD-55033 | ArrayExpress
| PRJNA251855 | ENA
| PRJNA238299 | ENA
2010-07-14 | E-GEOD-21943 | ArrayExpress
2010-07-14 | GSE21943 | GEO
2010-07-14 | E-GEOD-13110 | ArrayExpress
| GSE13110 | GEO
2015-08-07 | E-GEOD-60300 | ArrayExpress
2015-08-07 | E-GEOD-60299 | ArrayExpress