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Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35®

ABSTRACT: Investigation of whole genome gene expression level changes in Lcr35® pharmaceutical formulations, compared to the native strain.

ORGANISM(S): Lacticaseibacillus rhamnosus Lactobacillus

PROVIDER: GSE58820 | GEO | 2017/06/22

SECONDARY ACCESSION(S): PRJNA253585

REPOSITORIES: GEO

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Bile stress response in probiotic Lactobacillus rhamnosus GG

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Project description:Transcriptional profiling of probiotic Lactobacillus rhamnosus strain GG mid-exponential pH-controlled bioreactor cultures before and after exposure to bovine bile (0.2% ox gall). Keywords: bile, stress response Cell samples from four biological replicates were harvested right before (time point 0 min) and 10, 30 and 120 min after bile treatment. Each sample was compared to a common reference sample (time point 0 min, mid-exponential growth phase Lactobacillus rhamnosus GG cultures). A total of 12 hybridizations were performed using balanced dye-swap design. Dyes were balanced between compared sample pairs and between biological replicates.

2011-01-01 | E-GEOD-22536 | biostudies-arrayexpress