Dataset Information


Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma [expression]

ABSTRACT: Transcriptional profiling of two pediatric human DIPG cell lines, harboring the K27M mutation. DIPG cell lines were treated with GSKJ4 for 24 and 72 h. The untreated cell lines served as baseline, and expression fold changes in each treated cell line were determined relative to the baseline. Goal was to determine the effects of GSKJ4 treatment on global gene expression in DIPG cells harboring the K27M mutation. Overall design: Three-condition experiment: 72 h GSKJ4 treated cells vs. 24 h GSKJ4 treated cells vs. untreated cells. Biological replicates: 1 cell line, with 2 technical replicates per condition.

INSTRUMENT(S): Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)

SUBMITTER: Rintaro Hashizume  

PROVIDER: GSE59366 | GEO | 2014-07-14



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Pediatric brainstem gliomas often harbor oncogenic K27M mutation of histone H3.3. Here we show that GSKJ4 pharmacologic inhibition of K27 demethylase JMJD3 increases cellular H3K27 methylation in K27M tumor cells and demonstrate potent antitumor activity both in vitro against K27M cells and in vivo against K27M xenografts. Our results demonstrate that increasing H3K27 methylation by inhibiting K27 demethylase is a valid therapeutic strategy for treating K27M-expressing brainstem glioma. ...[more]

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