Transcriptomics,Genomics

Dataset Information

27

Effect of Glucocorticoids on microRNA Expression Profile of the Human Bone Microcirculatory Endothelial Cells from Femoral Head


ABSTRACT: Appropriate gene expression patterns form the basis for bone microcirculatory endothelial cells’ function and bone morphology. Although previous studies have elucidated the impact of hydrocortisone on bone microcirculatory endothelial cells’ specific gene expression, the exact differential transcriptomes and comprehensive gene expression profiles remain unknown. We have investigated the microRNA expression patterns before and after hydrocortisone administration of bone microcirculatory endothelial cells. Only 5 microRNAs were Benjamini-Hochberg characterized over 368 microRNAs candidates. Transcriptomic analysis of bone microcirculatory endothelial cells from human samples is highly informative due to their relevance to the large number of expressed genes. Our study provides a very valuable basis for investigation of genes, regulation and their co-expression network contributing to hydrocortisone induced disorders. Overall design: Two-condition experiment, hydrocortisone treated vs. untreated bone microcirculatory endothelial cells. Biological replicates: 8 control replicates, 8 treated replicates.

INSTRUMENT(S): Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (miRNA ID version)

SUBMITTER: Qingsheng Yu  

PROVIDER: GSE60093 | GEO | 2015-01-01

SECONDARY ACCESSION(S): PRJNA257459

REPOSITORIES: GEO

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Publications

[Preliminary study of impact of steroids on expression profile and transcriptome of bone microvascular endothelial cells].

Yu Qingsheng Q   Guo Wanshou W   Cheng Liming L   Lu Yufeng Y   Li Ping P  

Zhonghua yi xue za zhi 20141201 48


OBJECTIVE: To explore the differential expression profiles and transcriptomes of human bone microvascular endothelial cells via microarray and elucidate the molecular mechanisms underlying steroids-induced osteonecrosis. METHODS: Bone microvascular endothelial cells in cancellous bone of femoral head were harvested by type I collagenase and trypsin digestion and identified by immunofluorescent labeling with primary antibodies specific for vascular endothelial cells' biomarkers such as von Willeb  ...[more]

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