Transcriptomics,Genomics

Dataset Information

154

Zinc acquisition in Pseudomonas aeruginosa


ABSTRACT: This study addresses the impact of zinc limitation on the opportunistic human pathogen, Pseudomonas aeruginosa. Zinc limitation was assessed in the P. aeruginosa PAO1 strain using an isogenic deletion mutant lacking the periplasmic, zinc solute-binding protein, znuA (PA5498). ZnuA delivers bound zinc to its cognate ABC transporter, ZnuBC, for import into the cytoplasm. Our transcriptional analyses revealed P. aeruginosa to possess a multitude of zinc acquisition mechanisms, each of which were highly up-regulated in the zinc-deficient znuA mutant strain. P. aeruginosa also utilized zinc-independent paralogues of zinc-dependent genes to maintain cellular function under zinc limitation. Together, these data reveal the complex transcriptional response and versatility of P. aeruginosa to zinc depletion. Overall design: The WT (zinc replete) and znuA mutant strain (zinc limited) were grown in cation-defined media to mid-log phase prior to RNA isolation and analysis.

INSTRUMENT(S): Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1)

SUBMITTER: Bart Eijkelkamp  

PROVIDER: GSE60177 | GEO | 2017-01-01

SECONDARY ACCESSION(S): PRJNA257669

REPOSITORIES: GEO

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Publications


Pseudomonas aeruginosa is a ubiquitous environmental bacterium and a clinically significant opportunistic human pathogen. Central to the ability of P. aeruginosa to colonise both environmental and host niches is the acquisition of zinc. Here we show that P. aeruginosa PAO1 acquires zinc via an ATP-binding cassette (ABC) permease in which ZnuA is the high affinity, zinc-specific binding protein. Zinc uptake in Gram-negative organisms predominantly occurs via an ABC permease, and consistent with t  ...[more]

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