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Gene expression data of human dermal pilla cells, human induced pluripotent stem cell-derived CD271+CD90+ mesenchymal stem cells before and after the induction toward dermal papilla cells

ABSTRACT: To assess if human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) could be induce to acquire dermal papilla (DP) properties (especially hair inductive capacity), hiPSCs were initially programmed in serum-free MSC medium with FGF, TGFbeta and PDGF. Subsequently, hiMSCs were exposed to retinoic acid and activators of WNT, BMP and FGF signaling pathways. Global gene expression profiles were compared among primarily cultured human DP cells, hiPSC-MSCs before and after induction.

ORGANISM(S): Homo sapiens

PROVIDER: GSE61511 | GEO | 2015/01/01

SECONDARY ACCESSION(S): PRJNA261334

REPOSITORIES: GEO

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Gene expression data of human dermal pilla cells, human induced pluripotent stem cell-derived CD271+CD90+ mesenchymal stem cells before and after the induction toward dermal papilla cells

Project description:To assess if human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) could be induce to acquire dermal papilla (DP) properties (especially hair inductive capacity), hiPSCs were initially programmed in serum-free MSC medium with FGF, TGFbeta and PDGF. Subsequently, hiMSCs were exposed to retinoic acid and activators of WNT, BMP and FGF signaling pathways. Global gene expression profiles were compared among primarily cultured human DP cells, hiPSC-MSCs before and after induction. Human dermal papillae were microdissected and cultured from different donors. Induction from hiPSCs to MSCs and subsequent sorting of CD271+CD90+ subpopulation and thier induction to DP was performed using WD39 hiPSC lines in two independent experimentations to generate hiPSC-MSC-1,hiPSC-MSC-2 ipDPSC-1 and ipDPSC-2. Funding: The Human Stem Cells Informatization Project of the Ministry of Health, Labour and Welfare, Japan

2015-01-01 | E-GEOD-61511 | biostudies-arrayexpress

Gene expression in feather dermal papilla (DP)

Project description:The feather follicle is a “professional” regenerative organ that undergoes natural cycling and, regeneration after wound plucking. Similar to mammalian hair follicle, dermal papilla (DP) controls feather regeneration, shape, size, and axis. Here we report gene expression profiling for feather DP at different growth stages. For growth phase, we compared gene expression of DP, the ramogenic zone of feather branching epithelium (Erz) and the mesenchymal pulp (Pp). We also compared gene expression of DP at resting phase. To characterize the feather regeneration process, we further profiled gene expression at Day-2 and Day-4 post wound. Our results provide a resource for investigating feather growth and regeneration. Examination of gene expression in dermal papilla (DP) at growth phase and resting phase feather follicle, and during feather regeneration.

2014-01-22 | E-GEOD-42017 | biostudies-arrayexpress

Dermal papilla signature gene expression in immortalized human dermal papilla cells from balding and non-balding scalp

Project description:Transcriptome analysis of DP signature gene expression in hTERT-immortalized balding (BAB) and non-balding (BAN) dermal papilla cells derived from frontal and occipital scalp of male patients with androgenetic alopecia Hamilton grade IV.

2016-04-20 | GSE66663 | GEO

Gene expression in feather dermal papilla (DP)

2014-01-22 | GSE42017 | GEO

Epithelial stem cells co-cultured with the dermal papilla

Project description:Clonogenic keratinocyte stem cells isolated from the bulge area of human telogen follicles were co-cultured with dermal papilla cells in a transwell system. RNA was isolated from stem cells for different periods of time (day 0, 1, 2, and 5) after co-culture with DP and analyzed for changes in gene expression using Genechip microarrays. Keywords = epithelial stem cells Keywords = DP Keywords = co-culture Keywords: other

2004-06-10 | GSE1470 | GEO

High-throughput sequencing of extracellular vesicles from human dermal papilla cells

Project description:The use of dermal papilla cells for hair follicle (HF) regeneration is long accepted much attention. However, cultured dermal papilla cells tend to lose the hair-inducible capability during passaging, which restricts its application. Increasing evidences indicate that dermal papilla cells exert their regulatory function of HF growth mainly through their unique paracrine properties, opening up a way to exosome therapies.This study aimed to explore the effects of exosomes from high and low-passaged human scalp follicle dermal papilla cells (DP-Exos) on hair follicle stem cells (HFSCs) activation and hair growth, and to investigate the underline mechanism. DP-Exos were isolated by ultracentrifugation and cultured with human scalp follicles and HFSCs. The hair elongation and cell proliferation was assessed. Quantitative real-time PCR (qRT-PCR) and Western-blot were performed to detect the expression levels of a class of miRNAs and proteins which have positive roles in regulating hair growth and HFSCs proliferation. High throughput miRNA sequencing of miRNAs in high (P8) and low-passaged (P3) DP-Exos was performed, and the utmost miRNA and its target gene was identified via bioinformatics analysis.

2021-01-13 | GSE136241 | GEO

Expression data of freshly microdissected human hair follicle dermal papilla and its comparisons

2011-08-11 | GSE31324 | GEO

Transcription profiling of human epithelial stem cells co-cultured with the dermal papilla

2008-06-11 | E-GEOD-1470 | biostudies-arrayexpress

Expression data of freshly microdissected human hair follicle dermal papilla and its comparisons

Project description:The dermal papilla (DP) of the hair follicle plays crucial roles in the hair follcie morphogenesis and cycling. Thus, the elucication of human DP molecular signature is of great interest. DP cell culture by conventional method impairs intrinsic properties of DP cells. Isolatoion of human DP is hampered by the lack of specific cell surface markers. Thus, it still depends on manual microdissection, with which the removal of minor contamination is unfeasible. Cultured DP cells are mostly pure. Aggregation of cultured DP cells was shown to restore some intrinsic properties in cultured DP cells. Fibroblasts are distinct dermal cell population and provide baseline for DP arrays. Four sets of Genechip microarrays were generated from freshly-microdissected DPs (fDP), cultured DP cells (cDP), cultured fibroblasts (Fibro) collected from respective volunteers. Two sets of Genechips were additionally prepared from aggregated DP cells (agDP) and cultured DP cells which were used for the generation of agDPs (each set derived from respective volunteer). These Genechips can be compared to elucidate the genes possibly contribute to intrinsic property of intact human DPs. For instance, comparisons between cultured DP (cDP) and fibroblast (Fibro) or between aggregated DP (agDP) and cDP could sort out potential DP signature genes, as each population is predominantly consisted of DP cells. When the expression levels of those genes were compared between fDP and cDP chips, the genes up- or equally expressed in fDPs should represent DP signature genes.

2011-08-10 | E-GEOD-31324 | biostudies-arrayexpress

Effects of cinnamic acid on the hair growth ability of dermal papilla cells

Project description:We examined effects of cinnamic acid on the hair growth ability of dermal papilla cells. Treatment with cinnamic acid led to upregulation of G-protein coupled receptor signaling pathway and neuroactive ligand-receptor interaction in human DP cells.

2024-03-06 | GSE252536 | GEO

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