Transcriptomics,Genomics

Dataset Information

37

Genome-wide analysis of gene expression in DJ-1 deficient muscle cells


ABSTRACT: Excessive reactive oxygen species (ROS) underlie the pathogenesis of multiple disorders. Nevertheless, physiological levels of ROS are required for intracellular signalling and maintenance of metabolic homeostasis. DJ-1, a Parkinson’s disease-associated protein, is involved in the regulation of oxidative stress. Our aim in this study was to determine the effect of DJ-1 disruption on gene expression in muscle cells. To this end, we transfected a murine myoblast cell line, C2C12 cells with siRNA targeting DJ-1. Overall design: C2C12 myoblasts purchased from ATCC were transfected with scramble or DJ-1 siRNA, and 48 hours later, cells were induced to differentiate into myotubes. 4 days later, myotubes were harvested and processed for RNA extraction and hybridization.

INSTRUMENT(S): Illumina MouseWG-6 v2.0 expression beadchip

SUBMITTER: Minna Woo 

PROVIDER: GSE63051 | GEO | 2014-11-07

SECONDARY ACCESSION(S): PRJNA266518

REPOSITORIES: GEO

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Publications


Reactive oxygen species (ROS) have been linked to a wide variety of pathologies, including obesity and diabetes, but ROS also act as endogenous signalling molecules, regulating numerous biological processes. DJ-1 is one of the most evolutionarily conserved proteins across species, and mutations in DJ-1 have been linked to some cases of Parkinson's disease. Here we show that DJ-1 maintains cellular metabolic homeostasis via modulating ROS levels in murine skeletal muscles, revealing a role of DJ-  ...[more]

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