Project description:To exlore whether candidate target genes of certain miRNAs are differentially expressed in high-risk versus low-risk IPMN tissue. Through a pilot project, we identified miRNAs that differentiated between pathologically confirmed high-risk (invasive and high-grade) and low-risk (low- and moderate-grade) IPMN tissue. Using the current dataset, we explored the expression of gene targets that have been shown to be regulated by the identified miRNAs by integrating microarray expresssion data from 23 IPMNs (17 high-risk; 6 low-risk).
Project description:To discover a panel of mi(cro)RNAs that accurately differentiate between high-risk IPMN tissue (from pathologically-confirmed invasive or high-grade IPMN cases) and low-risk IPMN tissue (from pathologically-confirmed low-or moderate-grade IPMN cases). In a discovery phase, genome-wide miRNA expression profiling was performed on 28 surgically-resected, pathologically-confirmed IPMNs (19 high-risk, 9 low-risk) using Taqman Low Density Arrays. A validation phase was performed in 21 independent IPMNs (13 high-risk, 8 low-risk). We also explored genes regulated by the identified miRNAs by integrating microarray expression data from 23 IPMNs.