Dataset Information


Spring Varaemia of Carp Virus (SVCV) infection of adult zebrafish

ABSTRACT: During viral infection, a large number of immune response signaling molecules including the interferon regulatory (IRF) family and type I interferon (IFN) transcribe. The exact identity and expression levels of fish IRFs and type-I IFNs during viral infection remains largely unknown. Here, we utilized Illumina sequencing technology to determine differential expression patterns for both zebrafish IRFs and type-I IFNs during two stages of SVCV infection, i.e. 6h and 24h post-infection. For 12 zebrafish IRFs, we identified DrIRF1 mRNA as one of the most abundant in normal tissues and also in SVCV-infected tissues, but DrIRF11 had a very weak basal expression and was almost not induced by SVCV infection. We also identified the highly basal expression of DrIRF7, which together with DrIRF3, was highly induced by SVCV infection. For type-I IFNs, zebrafish has four IFN genes, three of which, IFN1/2/3, particularly IFN 1 and IFN 3, were significantly transcribed under the same conditions. Overall design: 12 adult healthy male and female (1:1) zebrafish were infected by intraperitoneal inoculation with approximately 50 μL SVCV (10-8TCID50/mL). 6 infected fish (male:female=1:1) were sacrificed at 6h post-injection, and 6 (male:female=1:1) were sacrificed at 24h post-injection for head kidney (6K and 24K) and spleen (6S and 24S). Head kidney and spleen (0K and 0S) from the 6 control adult zebrafish (male:female=1:1) were collected as negative control.

INSTRUMENT(S): Illumina HiSeq 2000 (Danio rerio)

SUBMITTER: Jianfang Gui  

PROVIDER: GSE63133 | GEO | 2014-11-11



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Zebrafish IRF1 regulates IFN antiviral response through binding to IFNϕ1 and IFNϕ3 promoters downstream of MyD88 signaling.

Feng Hui H   Zhang Yi-Bing YB   Zhang Qi-Min QM   Li Zhi Z   Zhang Qi-Ya QY   Gui Jian-Fang JF  

Journal of immunology (Baltimore, Md. : 1950) 20141222 3

In mammals, type I IFNs (mainly IFN-α/β) are primarily regulated by transcription factors of the IFN regulatory factor (IRF) family. Fish IFNs do not show a one-to-one orthologous relationship with mammalian type I IFN homologues. Using a bacterial one-hybrid reporter screening system and an overexpression approach to explore the molecular mechanism underlying fish IFN induction, we identified zebrafish Danio rerio IRF (DrIRF)1 as a positive regulator of the fish IFN antiviral response. Among 12  ...[more]

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