Dataset Information


Expression data of HSFA6b-overexpression and HSFA6b dominant-negative mutant lines of Arabidopsis after NaCl or Heat shock treatment

ABSTRACT: In order to study the improtance of HSFA6b in salt and heat stresses of arabidopsis, we generated the HSFA6b-overexpression (HSFA6b-OE) and dominant-negative (HSFA6b-RD) mutant lines. We used microarray to investigate how genes regulated by HSFA6b after NaCl or heat shock treatment. Overall design: Seven-day-old seedlings of wild type (Col-0), HSFA6b-overexpression (HSFA6b-OE) and HSFA6b dominant-negative (HSFA6b-RD) mutant lines grown at 22℃ on 0.5X MS plates containing 1% sucrose were treated with 150mM NaCl for 6hr or 37℃ heat shock for 1hr, with 22℃ treatment as a control. Subsequently, samples were collected for RNA extraction by use of the RNeasy Kit (Qiagen). The results were representative of two independent biological replicates for analysis.

INSTRUMENT(S): [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array

SUBMITTER: Ya-Chen Huang  

PROVIDER: GSE63372 | GEO | 2016-12-07



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The Heat Stress Factor HSFA6b Connects ABA Signaling and ABA-Mediated Heat Responses.

Huang Ya-Chen YC   Niu Chung-Yen CY   Yang Chen-Ru CR   Jinn Tsung-Luo TL  

Plant physiology 20160804 2

Heat stress response (HSR) is a conserved mechanism developed to increase the expression of heat shock proteins (HSPs) via a heat shock factor (HSF)-dependent mechanism. Signaling by the stress phytohormone abscisic acid (ABA) is involved in acquired thermotolerance as well. Analysis of Arabidopsis (Arabidopsis thaliana) microarray databases revealed that the expression of HSFA6b, a class A HSF, extensively increased with salinity, osmotic, and cold stresses, but not heat. Here, we show that HSF  ...[more]

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