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Detection and Characterization of Gene Expression Differences in Transgenic Glycine max Seeds with RNAseq

ABSTRACT: Transformation of Glycine max with seed-targeted expression vectors via Agrobacterium causes measurable unscripted gene expression changes in the seed transcriptome Overall design: mRNA was sequenced from three transgenic events expressing three different recombinant proteins in soybean seeds. Three plants were chosen from each as group replicates, and three seeds from each plant as individual biological replicates.

INSTRUMENT(S): Illumina HiSeq 2000 (Glycine max)

SUBMITTER: Kevin Chad Lambirth  

PROVIDER: GSE64620 | GEO | 2015-10-04



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A Comparison of transgenic and wild type soybean seeds: analysis of transcriptome profiles using RNA-Seq.

Lambirth Kevin C KC   Whaley Adam M AM   Blakley Ivory C IC   Schlueter Jessica A JA   Bost Kenneth L KL   Loraine Ann E AE   Piller Kenneth J KJ  

BMC biotechnology 20151001

<h4>Background</h4>Soybean (Glycine max) has been bred for thousands of years to produce seeds rich in protein for human and animal consumption, making them an appealing bioreactor for producing valuable recombinant proteins at high levels. However, the effects of expressing recombinant protein at high levels on bean physiology are not well understood. To address this, we investigated whether gene expression within transgenic soybean seed tissue is altered when large amounts of recombinant prote  ...[more]