Effects of Escherichia coli global gene expression due to microfluidic processing

ABSTRACT: Investigation in bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidic approaches offer ways of creating integrated point-of-care systems, analysing a sample from preparation, RNA isolation through to detection. Critical for on-chip diagnostics to deliver on their promise is that mRNA expression is not altered through the use microfluidic sample processing. Here, we investigate the impact on the use of a microfluidic sample processing system based on hydrodynamic separation upon RNA expression of bacteria isolated from blood to prove its suitability for further microfluidic test development.

ORGANISM(S): Escherichia coli Escherichia coli CFT073

PROVIDER: GSE68064 | GEO | 2016/04/01

SECONDARY ACCESSION(S): PRJNA281646

REPOSITORIES: GEO