Project description:mRNA profiling of three clival chordoma cell lines. The expression profiles were compared to previously published expression profiles of sacral chodomas (GEO accession number: GSE68497) to determine whether there are differences in expression that indicate stricing differences on molecular grounds.
Project description:Here, we used a proteomics approach to identify novel chordoma-specific cell-surface protein markers. Four established chordoma cell lines (U-CH17P, U-CH17M, U-CH17S and U-CH11R) were analyzed by quantitative proteomics using a comprehensive organellar fractionation approach based on differential ultracentrifugation. A subtractive proteomics strategy was applied to identify proteins that are plasma membrane enriched. The expression profiles of these cell-surface proteins were validated across chordoma cell lines, patient surgical tissue samples, and normal tissue lysates. The essentiality of these candidates was evaluated using chordoma cell line growth in vitro.
Project description:We report the DNA methylation profile of 4 chordoma cell lines (UCH1, UCH7, UM-Chor and MUG-Chor) and 1 ostesarcoma cell line (U2OS) using Illumina Infinium MethylationEPIC array
Project description:Chromatin profiling of chordoma collected by the Broad chordoma target discovery project paired end ATAC-Seq profiling in the UCH2 and MUGCHOR chordoma cell lines
Project description:mRNA profiling of three novel chordoma cell lines out of the same patient. The gene expression profiles of the primary tumour derived cell line U-CH17P was compared to the gene expression profiles of the metastases derived cell lines U-CH17S and U-CH17M in order to spot differently expressed genes, that are involved in the formation of metastases in chordoma.
Project description:TNF-α is an important inflammatory cytokine. Recent findings suggest tumor promoting inflammation could be a driving factor for chordoma progression. We used microarray to monitor the global changes in gene expression after TNF-α treatment for 1 year in two chordoma cell lines
Project description:TNF-α is an important inflammatory cytokine. Recent findings suggest tumor promoting inflammation could be a driving factor for chordoma progression. We used microarray to monitor the global changes in gene expression after TNF-α treatment for 1 week in two chordoma cell lines