Dataset Information

Organelle resolved proteomics reveals new chordoma cell surface markers required for proliferation and association with outcome

ABSTRACT: Here, we used a proteomics approach to identify novel chordoma-specific cell-surface protein markers. Four established chordoma cell lines (U-CH17P, U-CH17M, U-CH17S and U-CH11R) were analyzed by quantitative proteomics using a comprehensive organellar fractionation approach based on differential ultracentrifugation. A subtractive proteomics strategy was applied to identify proteins that are plasma membrane enriched. The expression profiles of these cell-surface proteins were validated across chordoma cell lines, patient surgical tissue samples, and normal tissue lysates. The essentiality of these candidates was evaluated using chordoma cell line growth in vitro.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Thomas Kislinger