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Changes in gene expression caused by loss of Med1 in the mouse prostate

ABSTRACT: MED1 is a transcriptional coactivator for gene-specific activators involved in growth and development, we were interested in identifying MED1 target genes potentially involved in prostate development by cDNA microarray. Med1 was conditional knocked out in mice prostate. We performed cDNA microarray with two sets: (1) RNA isolated from three WT ventral prostates and three MT ventral prostates; (2) RNA isolated from three WT lateral prostates and three MT lateral prostates. We used microarrays to detail the global programme of gene expression underlying how Med1 was involved in mice prostate development by regulating targeted genes expression

ORGANISM(S): Mus musculus

PROVIDER: GSE69124 | GEO | 2015/05/22

SECONDARY ACCESSION(S): PRJNA284607

REPOSITORIES: GEO

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Changes in gene expression caused by loss of Med1 in the mouse prostate

Project description:MED1 is a transcriptional coactivator for gene-specific activators involved in growth and development, we were interested in identifying MED1 target genes potentially involved in prostate development by cDNA microarray. Med1 was conditional knocked out in mice prostate. We performed cDNA microarray with two sets: (1) RNA isolated from three WT ventral prostates and three MT ventral prostates; (2) RNA isolated from three WT lateral prostates and three MT lateral prostates. We used microarrays to detail the global programme of gene expression underlying how Med1 was involved in mice prostate development by regulating targeted genes expression 12 mice prostate samples were divided into two groups: (1) three WT ventral prostates and three MT ventral prostates; (2) three WT lateral prostates and three MT lateral prostates.

2015-05-22 | E-GEOD-69124 | biostudies-arrayexpress

Transgenic expression of polyoma virus middle T antigen in the mouse prostate gives rise to carcinoma

Project description:Global gene expression profiles of ventral prostates of wild types and transgenic mice created with MT antigen normal prostate vs prostate cancer induced by MT expression

2011-06-30 | E-GEOD-26234 | biostudies-arrayexpress

Genes up and down regulated in LNCaP cells overexpressing MED1

Project description:To identify MED1 target genes involved in prostate tumorigenesis.

2012-09-26 | GSE41150 | GEO

Transgenic expression of polyoma virus middle T antigen in the mouse prostate gives rise to carcinoma

Project description:Global gene expression profiles of ventral prostates of wild types and transgenic mice created with MT antigen

2011-06-30 | GSE26234 | GEO

Genes up and down regulated in LNCaP cells overexpressing MED1

Project description:To identify MED1 target genes involved in prostate tumorigenesis. LNCap cells were transiently transfected with a MED1 expression vector or and empty vector control for 24 hours. Total RNA was extracted and gene microarray carried out. Experiment was performed in duplicate. Four samples total.

2012-09-26 | E-GEOD-41150 | biostudies-arrayexpress

CDK7 inhibition suppresses Castration-Resistant Prostate Cancer through MED1 inactivation

Project description:We performed RNA-seq and ChIP-seq in three prostate cell lines (VCaP, LNCaP and DU145) to ascertain the role of the mediator complex MED1 in AR signaling. Upon androgen stimulation, MED1 undergoes phosphorylation by CDK7 and physically engages with AR at super-enhancer sites, which is essential for AR-mediated transcription. The CDK7 specific inhibitor THZ1 blunts AR-dependent neoplastic growth by preventing the co-recruitment of AR/MED1 in a genome-wide fashion, and reverts the enzalutamide resistance characterized by hyper-phosphorylated MED1. The effect of THZ1 phenocopies that for MED1 and CDK7 knockdown.

2019-08-20 | GSE125245 | GEO

Med1 overexpression induces cell proliferation

2013-07-18 | GSE48950 | GEO

Phosphorylated MED1 Links Transcription Recycling and Cancer Growth

Project description:Mammalian RNA polymerase II (Pol II) initiation, elongation, termination and reinitiation are well studied, but how Pol II dynamically recycles after the transcription cycle remains unclear. By establishing in vitro and in vivo transcription recycling systems, we find that human Mediator 1 (MED1), when phosphorylated at the mammal-specific threonine 1032 by cyclin-dependent kinase 9, dynamically travels with Pol II throughout the transcribed genes to drive Pol II recycling. Mechanistically, MED1 phosphorylation leads to an increase of recycled Pol II via the molecular bridge of MED31, enhancing mRNA output during the transcription recycling process. Importantly, MED1 phosphorylation increases during prostate cancer progression to the lethal phase, and pharmacological inhibition of CDK9 decreases prostate tumor growth through decreasing MED1 phosphorylation and Pol II recycling. Our findings reveal essential mechanisms underlying Pol II recycling and suggest a neglected, yet fundamental Pol II transcription process for therapeutic intervention.

2022-04-20 | GSE127894 | GEO

Med1 overexpression induces cell proliferation

Project description:Med1 overexpression leads to induction of a wide spectrum of genes. Adenovirally-driven overexpression of Med1 in mouse liver stimulates hepatocyte DNA synthesis with enhanced expression of DNA replication, cell cycle control and liver specific genes as observed at day 3 and day 5 post injection. Med1 gene is amplified in a number of cancers so in this study we tested the hypothesis that Med1 by itself has the capacity to induce cell proliferation. Analysis of the Med1- induced gene expression showed a robust induction of a wide spectrum of genes involved in hepatocellular proliferation. Med1 floxed mice (Med1fl/fl) were injected with Ad-His-Med1 (adenovirus expressing Med1 gene) via tail vein and killed 3 or 5 days after injection. Ad-LacZ (adenovirus expression beta-galactosidase gene) injected mouse liver served as control. Total RNA were isolated from the liver and subjected to the microarray.

2013-07-18 | E-GEOD-48950 | biostudies-arrayexpress

A constitutively activated form of the p110 beta isoform of PI3-kinase induces prostatic intraepithelial neoplasia in mice

Project description:The global gene expression profiles of ventral prostates of wild type mice and p110 beta transgenic mice. normal prostate vs. prostate cancer induced by the overexpression of the active allele of p110 beta

2010-06-08 | E-GEOD-21543 | biostudies-arrayexpress

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