Transcriptomics,Genomics

Dataset Information

152

RNA-sequencing of EpH4 and EpRas cells treated with Etv4 and Etv5 siRNAs and/or TGF-beta.


ABSTRACT: We performed RNA-seq analysis in mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) to analyze the mechanisms of transcriptional regulation during TGF-beta-induced EMT. Overall design: RNA-seq was perfomed using EpH4 and EpRas cells transfected with Etv4 and Etv5 siRNAs and stimulated with 1 ng/ml TGF-beta or left untreated for 48 h.

INSTRUMENT(S): Ion Torrent Proton (Mus musculus)

SUBMITTER: Daizo Koinuma  

PROVIDER: GSE69363 | GEO | 2017-04-10

SECONDARY ACCESSION(S): PRJNA285319

REPOSITORIES: GEO

altmetric image

Publications

Dynamics of chromatin accessibility during TGF-β-induced EMT of Ras-transformed mammary gland epithelial cells.

Arase Mayu M   Tamura Yusuke Y   Kawasaki Natsumi N   Isogaya Kazunobu K   Nakaki Ryo R   Mizutani Anna A   Tsutsumi Shuichi S   Aburatani Hiroyuki H   Miyazono Kohei K   Koinuma Daizo D  

Scientific reports 20170426 1


Epithelial-mesenchymal transition (EMT) is induced by transforming growth factor (TGF)-β and facilitates tumor progression. We here performed global mapping of accessible chromatin in the mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) using formaldehyde-assisted isolation of regulatory element (FAIRE)-sequencing. TGF-β and Ras altered chromatin accessibility either cooperatively or independently, and AP1, ETS, and RUNX binding motifs were enriched in the  ...[more]

Similar Datasets

| GSE94977 | GEO
| GSE89618 | GEO
| GSE69387 | GEO
| GSE69364 | GEO
2012-02-01 | E-GEOD-33997 | ArrayExpress
2009-10-02 | E-GEOD-18267 | ArrayExpress
| GSE47225 | GEO
2011-03-24 | E-GEOD-21209 | ArrayExpress
2011-03-25 | GSE21209 | GEO
2013-06-03 | E-GEOD-34833 | ArrayExpress