Dataset Information


RNA-sequencing of EpH4 and EpRas cells treated with Etv4 and Etv5 siRNAs and/or TGF-beta.

ABSTRACT: We performed RNA-seq analysis in mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) to analyze the mechanisms of transcriptional regulation during TGF-beta-induced EMT. Overall design: RNA-seq was perfomed using EpH4 and EpRas cells transfected with Etv4 and Etv5 siRNAs and stimulated with 1 ng/ml TGF-beta or left untreated for 48 h.

INSTRUMENT(S): Ion Torrent Proton (Mus musculus)

SUBMITTER: Daizo Koinuma  

PROVIDER: GSE69363 | GEO | 2017-04-10



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Dynamics of chromatin accessibility during TGF-β-induced EMT of Ras-transformed mammary gland epithelial cells.

Arase Mayu M   Tamura Yusuke Y   Kawasaki Natsumi N   Isogaya Kazunobu K   Nakaki Ryo R   Mizutani Anna A   Tsutsumi Shuichi S   Aburatani Hiroyuki H   Miyazono Kohei K   Koinuma Daizo D  

Scientific reports 20170426 1

Epithelial-mesenchymal transition (EMT) is induced by transforming growth factor (TGF)-β and facilitates tumor progression. We here performed global mapping of accessible chromatin in the mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) using formaldehyde-assisted isolation of regulatory element (FAIRE)-sequencing. TGF-β and Ras altered chromatin accessibility either cooperatively or independently, and AP1, ETS, and RUNX binding motifs were enriched in the  ...[more]

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