Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

C1 analysis using mixtures of human (HEK) and mouse (3T3) cells

ABSTRACT: A cell supsension containing an equal mix of HEK and 3T3 cells was used in the Fluidigm C1

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE70151 | GEO | 2015/06/24

SECONDARY ACCESSION(S): PRJNA287748

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 50 cells per microliter

Project description:A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. The cells were mixed at a final concentration of 50 cells per microliter.

2015-05-20 | GSE63269 | GEO

Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 12.5 cells per microliter

Project description:A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. Cells were mixed at a final concentration of 12.5 cells per microliter in droplets.

2015-05-20 | GSE66693 | GEO

Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 12.5 cells per microliter

2015-05-20 | E-GEOD-66693 | biostudies-arrayexpress

C1 analysis using mixtures of human (HEK) and mouse (3T3) cells

Project description:C1 analysis using mixtures of human (HEK) and mouse (3T3) cells

| PRJNA287748 | ENA

Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells

Project description:This study provides an assessment of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells. The system combines microfluidic technology and nanoliter-scale reactions. We sequenced 622 cells allowing identification of 341 islet cells with high-quality gene expression profiles. The cells clustered into populations of alpha-cells (5%), beta-cells (92%), delta-cells (1%) and PP-cells (2%). We identified cell-type specific transcription factors and pathways primarily involved in nutrient sensing and oxidation and cell signaling. Unexpectedly, 281 cells had to be removed from the analysis due to low viability (23%), low sequencing quality (13%) or contamination resulting in the detection of more than one islet hormone (64%). Collectively, we provide a resource for identification of high-quality gene expression datasets to help expand insights into genes and pathways characterizing islet cell types. We reveal limitations in the C1 Fluidigm cell capture process resulting in contaminated cells with altered gene expression patterns. This calls for caution when interpreting single-cell transcriptomics data using the C1 Fluidigm system.

2016-02-19 | GSE77980 | GEO

Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells

2016-02-19 | E-GEOD-77980 | biostudies-arrayexpress

C1 single-cell RNA-seq of Adult ID4-EGFP mouse spermatoognia

Project description:To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from Adult ID4-EGFP+ spermatogonia were subdivided into subpopulations that displayed distinct fates when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq.

2018-11-06 | GSE108974 | GEO

C1 single-cell RNA-seq of immature (P6) ID4-EGFP mouse spermatoognia

Project description:To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from P6 ID4-EGFP+ spermatogonia were subdivided into subpopulations that displayed distinct fates when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq.

2018-11-06 | GSE108970 | GEO

C1 single-cell RNA-seq of Adult Human spermatoognia

Project description:To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from Adult Human spermatogonia were subdivided into subpopulations based on the levels of ID4 mRNA (determined in this experiment). This correlates with distinct fates of corresponding mouse spermatogonia when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq.

2018-11-06 | GSE108977 | GEO

Single Cell RNA-seq data of C1-sgControl, C1-sgEED and C1-sgKMT2D cells

Project description:Analysis of gene expression patterns at single cell level of C1-sgControl, C1-sgEED and C1-sgKMT2D cells.

2022-04-26 | GSE201441 | GEO