Transcriptomics,Genomics

Dataset Information

29

Altered RNA expression in parenteral nutrition mouse model


ABSTRACT: We reported altered RNA expression profiles in wild-type (WT) C57BL/6J mice upon parenteral nutrition treatment compared to saline infusion controls. RNA samples used were isolated from the livers of PN or saline infused mice. RNA samples from 3 saline or 3 PN mouse livers were pooled, respectively. Final fold change were calculated by normalize the signal intensity of each probe in PN sample to that of the saline sample. Overall design: On day 1, jugular vein catheterization survival surgery was performed on mouse (age 6-10 weeks).On day 2, the catheter was connected to an infusion line and the mouse was transferred to a metabolic cage. Surgical mice then started to receive saline infusion. On day 3, surgical mice started receiving either parenteral nutrion (PN), or saline infusion as control. Food was removed from the cage for mice in the PN group. Mice in saline group continue to have access to food. On day 11, PN and saline control mice were sacrificed and tissues (liver, ileum and colon) were collected for RNA isolation. After RT-qPCR verification, RNA samples from the same tissues from 3 PN mice and 3 saline mice were pooled, respectively. Pooled RNA samples for liver, ileum and colon tissues were then used for microarray analysis. Please note that only liver samples have been submitted and that sample data table contains the data filtered by 1.5-fold cutoff.

INSTRUMENT(S): [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]

SUBMITTER: Le Zhan  

PROVIDER: GSE71286 | GEO | 2016-02-01

SECONDARY ACCESSION(S): PRJNA290810

REPOSITORIES: GEO

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Publications

Dysregulation of bile acid homeostasis in parenteral nutrition mouse model.

Zhan Le L   Yang Ill I   Kong Bo B   Shen Jianliang J   Gorczyca Ludwik L   Memon Naureen N   Buckley Brian T BT   Guo Grace L GL  

American journal of physiology. Gastrointestinal and liver physiology 20151112 2


Long-term parenteral nutrition (PN) administration can lead to PN-associated liver diseases (PNALD). Although multiple risk factors have been identified for PNALD, to date, the roles of bile acids (BAs) and the pathways involved in BA homeostasis in the development and progression of PNALD are still unclear. We have established a mouse PN model with IV infusion of PN solution containing soybean oil-based lipid emulsion (SOLE). Our results showed that PN altered the expression of genes involved i  ...[more]

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