Genomics

Dataset Information

47

Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism


ABSTRACT: The neuroectoderm is patterned along a rostral-caudal axis in response to localized factors in the embryo, but exactly how these factors act as positional information for this patterning is not yet fully understood. Here, using the self-organizing properties of mouse embryonic stem cell (ESC), we report that ESC-derived neuroectoderm self-generates a Six3+ rostral and a Irx3+ caudal bipolarized patterning. In this instance, localized Fgf signaling performs dual roles, as it regulates Six3+ rostral polarization at an earlier stage and promotes Wnt signaling at a later stage. The Wnt signaling components are differentially expressed in the polarized tissues, leading to genome-wide Irx3+ caudal-polarization signals. Surprisingly, differentially expressed Wnt agonists and antagonists have essential roles in orchestrating the formation of a balanced rostral-caudal neuroectoderm pattern. Together, our findings provide key processes for dynamic self-patterning and evidence that a temporally and locally regulated interaction between Fgf and Wnt signaling controls self-patterning in ESC-derived neuroectoderm. Overall design: The duplicated samples for each of Rax::GFP+ and Rax::GFP- cells during ESC (embryonic stem cell)-derived neural tissue development were analyzed.

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

SUBMITTER: NOZOMU TAKATA  

PROVIDER: GSE72520 | GEO | 2017-08-05

SECONDARY ACCESSION(S): PRJNA294258

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE72520_RAW.tar Raw
filelist.txt Txt
Items per page:
1 - 2 of 2
altmetric image

Publications

Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.

Takata Nozomu N   Sakakura Eriko E   Eiraku Mototsugu M   Kasukawa Takeya T   Sasai Yoshiki Y  

Nature communications 20171107 1


The neuroectoderm is patterned along a rostral-caudal axis in response to localized factors in the embryo, but exactly how these factors act as positional information for this patterning is not yet fully understood. Here, using the self-organizing properties of mouse embryonic stem cell (ESC), we report that ESC-derived neuroectoderm self-generates a Six3<sup>+</sup> rostral and a Irx3<sup>+</sup> caudal bipolarized patterning. In this instance, localized Fgf signaling performs dual roles, as it  ...[more]

Similar Datasets

2017-01-01 | S-EPMC5673904 | BioStudies
1000-01-01 | S-EPMC4833820 | BioStudies
2008-01-01 | S-EPMC2575295 | BioStudies
2011-01-01 | S-EPMC3155607 | BioStudies
1000-01-01 | S-EPMC4915947 | BioStudies
2018-01-01 | S-EPMC5868256 | BioStudies
2016-01-01 | S-EPMC4909856 | BioStudies
1000-01-01 | S-EPMC3799351 | BioStudies
2008-01-01 | S-EPMC2229838 | BioStudies
2017-01-01 | S-EPMC5728169 | BioStudies