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Hypothalamic reprogramming by sperm microRNA

ABSTRACT: Nine microRNAs (miRs) previously identified as increased in the sperm of stressed sires were microinjected into C57/Bl6:129S6/SvEvTac mouse single cell zygotes to examine the hypothesis that specific sperm miRs function postfertilization to alter offspring stress responsivity. Derived mice were exmined for hypothalamic-pituitary-adrenal axis stress response in adulthood, and the paraventricular nucleus of the hypothalamus was subsequently collected for gene expression analysis by next gen sequencing. Similar to what we reported previously in our paternal stress model, the majority of diffferenitally expressed genes in the PVN exhibited decreased expression, supporting that an increase of specific sperm miRs in the zygote can elicit long-term genetic reprogramming. Futher, marked changes in the expression of extracellular matrix and collagen gene sets suggested altered blood-brain barrier permeability with potential consequences for neuroendocrine function.

ORGANISM(S): Mus musculus

PROVIDER: GSE73574 | GEO | 2016/01/01

SECONDARY ACCESSION(S): PRJNA297350

REPOSITORIES: GEO

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Hypothalamic reprogramming by sperm microRNA

Project description:Nine microRNAs (miRs) previously identified as increased in the sperm of stressed sires were microinjected into C57/Bl6:129S6/SvEvTac mouse single cell zygotes to examine the hypothesis that specific sperm miRs function postfertilization to alter offspring stress responsivity. Derived mice were exmined for hypothalamic-pituitary-adrenal axis stress response in adulthood, and the paraventricular nucleus of the hypothalamus was subsequently collected for gene expression analysis by next gen sequencing. Similar to what we reported previously in our paternal stress model, the majority of diffferenitally expressed genes in the PVN exhibited decreased expression, supporting that an increase of specific sperm miRs in the zygote can elicit long-term genetic reprogramming. Futher, marked changes in the expression of extracellular matrix and collagen gene sets suggested altered blood-brain barrier permeability with potential consequences for neuroendocrine function. mRNA profiling of paraventricular nucleus from adult male mice derived from zygote microinjection of nine miRs (multi-miR; miR-29, miR-30a, miR-30c, miR-32, miR-193-5p, miR-204, miR-375, miR-532-3p, miR-698) or PBS. Six biological replicates per group (12 total cDNA libraries) were multiplexed and sequenced on two identical HiSeq2000 lanes (Illumina).

2016-07-03 | E-GEOD-73574 | biostudies-arrayexpress

Convergence of distinct RNA-silencing pathways on GW182/Tnrc6 II

Project description:RNA-sequencing in mouse embryonic stem cells: Ctrl: GFP_5xT71BS (mES [129S6/SvEvTac], yGlob [GFP_5xT71BS]. Trim71-/-: GFP_5xT71BS (mES [129S6/SvEvTac], yGlob [GFP_5xT71BS] Trim71 -/-. Note pervasive gene expression changes in samples with TNRC6A overexpression in comparison with samples with CAS9 overexpression.

2024-02-13 | GSE231730 | GEO

Project description:Mus musculus 129S6/SvEvTac Genome Sequencing

| PRJNA817075 | ENA

Infection of macrophages with functional Slc11a1 yields differences in global gene expression, regulation of metal ions, and infection outcomes

Project description:Manipulation of zinc at the host-pathogen interface depends on both the identity of the pathogen and the nature of the host cell. In this study, we examine the response of bone marrow derived macrophages from 129S6/SvEvTac mice to infection by Salmonella Typhimurium. Unlike Balb/c and C57BL/6 mice, 129S6/SvEvTac mice possess a functional Slc11a1 (Nramp-1), a phagosomal transporter of divalent cations. We examine the changes in gene expression upon treatment with live or heat killed Salmonella at 2 Hrs and 18 Hrs post infection, and observed widespread changes in metal transport, metal-dependent, and metal homeostasis genes, suggesting significant remodeling of iron, copper, and zinc availability by host cells.

2021-02-12 | GSE166642 | GEO

Liver miRNA profiling in iron deficient and overload mice

Project description:8-week-old wild-type 129S6/SvEvTac mice were maintained under regular iron diet (containing 330 ppm iron) or iron-enriched diet (the same standard diet supplemented with 2% carbonyl iron) for 4 weeks. 7-week-old wild-type 129S6/SvEvTac mice were maintained under iron-deficient diet (with negligible iron content) for 5 weeks. All mice were sacrificed at the age of 12 weeks. Livers were harvested and total RNA was extracted using TRIZol method followed by miRNA-enrichment using Qiagen columns. Liver RNA samples from 3 mice (female and male) from each group were sent to Exiqon for microarray analysis.

2014-09-04 | E-MTAB-1520 | biostudies-arrayexpress

Transcription profiling of mouse duodenum and liver with primary (Hfe ?/? and C282Y homozygous mice) and secondary iron overload vs 129S6/SvEvTac wild type controls

Project description:We determined duodenal and liver gene response patterns in mice with primary (Hfe ?/? and C282Y homozygous mice) and secondary iron overload versus 129S6/SvEvTac wild type controls.

2013-01-30 | E-MEXP-5 | biostudies-arrayexpress

Sire modulates developmental kinetics and transcriptome in the bovine embryo

Project description:The impact of sire on pre-implantation embryonic development in cattle remains poorly understood. This study evaluated differences in embryos produced from sires with varying capacities to produce blastocysts. Sires classified as high (HP) and low-performing (LP) based on their ability to produce embryos were used in order to better understand how sire regulates embryonic development. By monitoring embryo development, it was determined that the most common arrest stage was the 5-6 cell stage. Embryos (4-6 cells) from HP and LP sires were analyzed for autophagic activity, where embryos for LP sires exhibited increased autophagy than HP-derived embryos. Transcriptome analysis of 4-cell embryos found that embryos from LP sires might have issues in sperm mitochondrial clearance, histone retention, and DNA damage, while HP sires have increased expression of genes involved in transcription, chromosome segregation, and cell division. In conclusion, LP sires have an increased proportion of embryos arresting at the 5-6 cell stage, and these embryos have higher rates of cellular stress due to paternal contributions from the spermatozoon.

2024-01-01 | GSE223383 | GEO

Expression data from thioglycollate-elicited peritoneal macrophages from 129S6/SvEvTac and DBA2/J mice

Project description:Strain differences influence susceptibility to atherosclerosis. Apolipoprotein E-null mice on a DBA/2J genetic background (DBA-apoE) are highly susceptible to atherosclerosis in the aortic root area compared with those on a 129S6/SvEvTac background (129-apoE). To explore strain-specific differences affecting the susceptibility to atherosclerosis, we performed microarray analysis of macrophages from wild type mice of each strains.

2014-12-01 | GSE52473 | GEO

Expression data from thioglycollate-elicited peritoneal macrophages from 129S6/SvEvTac and C57BL/6 mice

Project description:Strain differences influence susceptibility to atherosclerosis. Apolipoprotein E-null mice on a C57BL/6 genetic background (B6-apoE) are highly susceptible to atherosclerosis in the aortic root area compared with those on a 129S6/SvEvTac background (129-apoE). To explore strain-specific differences affecting the susceptibility to atherosclerosis, we performed microarray analysis of macrophages from wild type mice of each strains.

2014-12-01 | GSE52816 | GEO

Strain-specific differences in brain gene expression in a hydrocephalic mouse model with motile cilia dysfunction

Project description:Analysis of strain-specific differences in gene expression in brains from a hydrocephalic mouse model of primary ciliary dyskinesia. The results identify genes that are differentially expressed between C57BL6/J and 129S6/SvEvTac brains. These genes encode proteins that function in a variety of cellular processes and include some that are relevant to hydrocephalus and cilia function, providing insight into the mechanisms underlying susceptibility to hydrocephalus.

2018-09-06 | GSE113233 | GEO

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