Dataset Information


82-kDa ChAT and SATB1 ChIP-seq after vehicle or amyloid-beta exposure

ABSTRACT: Using chromatin immunoprecipitation and next-generation sequencing (ChIP-seq), we assessed the effects of acute exposure to oligomeric amyloid-beta on 82-kDa ChAT and SATB1 genome association in human SH-SY5Y neural cells, finding that Aβ-exposure increased 82-kDa ChAT and SATB1 association with gene promoters, introns and matrix attachment regions. We found that both SATB1 and 82-kDa ChAT associate with synapse and cell stress related genes after amyloid-beta exposure. Overall design: Examination of effects of amyloid-beta exposure on 82-kDa ChAT and SATB1 in human SH-SY5Y neural cells

INSTRUMENT(S): Illumina Genome Analyzer (Homo sapiens)

SUBMITTER: Sandeep K Gill 

PROVIDER: GSE73576 | GEO | 2016-04-11



altmetric image


82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.

Winick-Ng Warren W   Caetano Fabiana A FA   Winick-Ng Jennifer J   Morey Trevor M TM   Heit Bryan B   Rylett R Jane RJ  

Scientific reports 20160407

The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1-42 (Aβ1-42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1-42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generati  ...[more]

Similar Datasets

2008-06-12 | E-GEOD-3506 | ArrayExpress
| PRJNA297352 | ENA
2012-05-01 | GSE37661 | GEO
| GSE23000 | GEO
2009-06-01 | E-MEXP-1913 | ArrayExpress
| PRJNA93543 | ENA
2015-04-08 | E-GEOD-67643 | ArrayExpress
2015-10-22 | E-MEXP-1913 | ExpressionAtlas
| GSE22525 | GEO
2009-09-04 | E-GEOD-9169 | ArrayExpress