Genomics

Dataset Information

143

82-kDa ChAT and SATB1 ChIP-seq after vehicle or amyloid-beta exposure


ABSTRACT: Using chromatin immunoprecipitation and next-generation sequencing (ChIP-seq), we assessed the effects of acute exposure to oligomeric amyloid-beta on 82-kDa ChAT and SATB1 genome association in human SH-SY5Y neural cells, finding that Aβ-exposure increased 82-kDa ChAT and SATB1 association with gene promoters, introns and matrix attachment regions. We found that both SATB1 and 82-kDa ChAT associate with synapse and cell stress related genes after amyloid-beta exposure. Overall design: Examination of effects of amyloid-beta exposure on 82-kDa ChAT and SATB1 in human SH-SY5Y neural cells

INSTRUMENT(S): Illumina Genome Analyzer (Homo sapiens)

SUBMITTER: Sandeep K Gill 

PROVIDER: GSE73576 | GEO | 2016-04-11

SECONDARY ACCESSION(S): PRJNA297352

REPOSITORIES: GEO

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Publications

82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.

Winick-Ng Warren W   Caetano Fabiana A FA   Winick-Ng Jennifer J   Morey Trevor M TM   Heit Bryan B   Rylett R Jane RJ  

Scientific reports 20160407


The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1-42 (Aβ1-42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1-42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generati  ...[more]

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