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RNA-Seq of human astrocytes

ABSTRACT: Astrocytes were purified from fetal and adult human brain tissue using an immunopanning method with the HepaCAM antibody. Samples were taken from otherwise 'healthy' pieces of tissue, unless otherwise specified.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE73721 | GEO | 2015/12/11

SECONDARY ACCESSION(S): PRJNA297760

REPOSITORIES: GEO

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RNA-Seq of human astrocytes

Project description:Astrocytes were purified from fetal and adult human brain tissue using an immunopanning method with the HepaCAM antibody. Samples were taken from otherwise 'healthy' pieces of tissue, unless otherwise specified. 6 fetal astrocyte samples, 12 adult astrocyte samples, 8 GBM or sclerotic hippocampal samples, 4 whole human cortex samples, 4 adult mouse astrocyte samples, and 11 human samples of other purified CNS cell types

2015-12-11 | E-GEOD-73721 | biostudies-arrayexpress

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Project description:Astrocytes were purified from mouse cortices and human cortex tissue by immunopanning with HepaCAM. All treatments were proceeded on 3DIV in serum-free medium. Astrocytes were treated by TNFα for 48hr, by Poly I:C for 72hr, or by hypoxia for 72hr. Acutely purified atrocytes RNA was harvest immediately after HepaCAM immunopanning. Serum selected astrocytes RNA was collected after 4-6 days culturing in serum medium. Serum-free cultured astrocytes RNA was collected on 5-6 DIV. Serum-selection purified human astrocytes were transplanted into P2-11 Rag2 immunodeficient mouse brains, and after about 8 months, transplanted human and host mouse astocytes RNA was collected acutely on HepaCAM immunopanning dish.

2021-04-21 | GSE147870 | GEO

Human Astrocytes Exhibit Tumor Microenvironment, Age- and Sex-Related Transcriptomic Signatures

Project description:Human astrocytes were purified from cortical surgical resections from consenting patients; Astrocytes were purified by immunopanning with HepaCAM. Total RNA of purified astrocytes was isolated and sequenced by Illumina HiSeq 4000.

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Project description:Astrocytes were purified from postnatal day 2 mouse cortices by immunopanning with HepaCAM. Inhibitors and DMSO were added on in-vitro day 2. HBEGF was depleted on the cell prep day and till in-vitro day 7. In-vitro day 2, 7 and 14 samples were collected on designed timepointed.

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Project description:Single-cell RNA-seq: We used single-cell RNAseq to investigate the maturation of astrocytes within human cortical spheroids Bulk RNA-seq: Bulk sequencing from astrocytes and neurons purified (via immunopanning) from iPSC-derived coritical spheroids at varying in vitro differentiation states

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Expression analysis of astrocytes isolated from the rat forebrain

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Bulk RNA-seq of immunopanned astrocytes with the treatment of Shh and Fgf

Project description:Purpose: Examine differential gene expression changes in in vitro astrocytes with the administration of Shh agonist and Fgf protein vs control; Methods: Astrocytes were purified using a previously published immunopanning protocol, SAG-Shh agonist and DMSO are added into cell culture medium for 3 days, and Fgf2 and Fgf10 were added to medium respectively for 7 days, PBS treated astrocytes as controls. RNA-seq were perfomed (Lexongen QuantSeq3'mRNA-SEQ V2 Library prep kit) on all different conditions; Results: We observed differential gene expression changes in SAG treated conditions compared to WT, but did not observe a significant number of genes changes in Fgf treated groups compared to controls.

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Expression analysis of astrocytes isolated from the rat forebrain

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Regionally specified human pluripotent stem cell-derived astrocytes

Project description:Bulk RNA-seq of highly pure regionally specified human pluripotent stem cell-derived astrocytes

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HepaCAM knockdown impairs learning and memory in mice by regulating cholesterol synthesis pathway

Project description:Astrocytes engage in metabolic interactions with nearby neurons by supplying various substances. However, how they regulate learning and memory through changes in the metabolic state of neurons is still unclear. In this study, we show that knocking down astrocytic hepaCAM silences genes related to cholesterol biosynthesis in astrocytes, leading to a decrease in cholesterol levels. Additionally, astrocytes play a crucial role in synapse development and function by releasing signals that guide neurons. Consequently, without enough cholesterol, the level of neuronal synaptic proteins and the density of dendritic spines decrease. Then we demonstrate that knocking down hepaCAM leads to impaired learning and memory in mice. Collectively, hepaCAM, a cell adhesion molecules specifically expressed in astrocytes, regulates learning and memory by regulating the cholesterol synthesis pathway.

2025-07-01 | GSE280264 | GEO

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