Genomics

Dataset Information

52

Expression data from intestinal mucosa of Zucker rats


ABSTRACT: Obesity is a chronic, complex and multifactorial disease that has reached pandemia levels and is becoming a serious health problem. Intestinal microbiota is considered a main factor that affects body weight and fat mass, which points toward a critical role in the development of obesity. In this sense, probiotic bacteria might modulate the intestinal microbiota and the mucosal-associated lymphoid tissue. The aim of this study was to investigate the effects of L. paracasei, L. rhamnosus and B. breve feeding on the intestinal mucosa gene expression in a genetic animal model of obesity. We used microarrays to investigate the global gene expression on intestinal mucosa after the treatment with probiotic strains. Overall design: Forty-eight Zucker-Leprfa/fa and 16 Zucker-lean+/fa male rats weighing 168-180 g were used. After 5 days of adaptation, 8 Zucker-lean+/fa (lean reference) and 8 Zucker-Leprfa/fa rats (obese reference) were euthanized as a reference. The remaining 40 Zucker-Leprfa/fa rats were then randomly assigned to receive 10^10 CFUs of one of the three probiotic strains (probiotic strains groups), or a placebo (obese placebo) by oral administration each day for 30 days. An additional group of 8 Zucker-lean+/fa rats (control placebo) received placebo. Samples of intestinal mucosa were taken and RNA isolated to carry out a differential gene expression study. with an Affymetrix microarray (Rat Gene 1.1 ST Array Plate).

INSTRUMENT(S): [RaGene-1_1-st] Affymetrix Rat Gene 1.1 ST Array [transcript (gene) version]

SUBMITTER: Julio Ramon Plaza Diaz  

PROVIDER: GSE73848 | GEO | 2016-12-25

SECONDARY ACCESSION(S): PRJNA298220

REPOSITORIES: GEO

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Publications

Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains.

Plaza-Díaz Julio J   Robles-Sánchez Cándido C   Abadía-Molina Francisco F   Morón-Calvente Virginia V   Sáez-Lara María José MJ   Ruiz-Bravo Alfonso A   Jiménez-Valera María M   Gil Ángel Á   Gómez-Llorente Carolina C   Fontana Luis L  

Scientific reports 20170516 1


We have previously reported that administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 to obese Zucker-Lepr fa/fa rats attenuates liver steatosis and exerts anti-inflammatory effects. The goal of the present work was to investigate the modulation of gene expression in intestinal mucosa samples of obese Zucker-Lepr fa/fa rats fed the probiotic strains using a DNA microarray and postgenomic techniques. We also measured secr  ...[more]

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