Transcriptomics,Genomics

Dataset Information

154

GATA2/eGFP knockin study to trace the endothelial to hematopoietic transition and hematopoietic progenitor cells in human hematopoiesis.


ABSTRACT: We generated GATA2/eGFP knockin human embryonic stem cells (H1-GATA2W/eGFP hESCs) and analyzed eGFP expression at different hematopoietic stages, paritcularly the hemogenic endothelium (HE) and hematopoietic progenitor cell (HPC) stage. Our results revealed that, at the HE stage, eGFP expression discriminates authentic HE from non-hematopoietic endothlial cells (EC) and, at the HPC stage, eGFP expression marks the HPC. Further transcriptome comparison analysis revealed distinct characteristics of the HE and EC. Our study provided ideal model to study the EHT and HPC generation, therefore underpining further study on human ESC modeled hematopoiesis. Overall design: We compared the transcriptome of eGFP+CD34+CD31+CD43- hemogenic endothelium and eGFP-CD34+CD31+CD43- endothelial cells derived from GATA2w/eGFP hESCs by RNA-Seq.

INSTRUMENT(S): Illumina MiSeq (Homo sapiens)

SUBMITTER: Hao Sun  

PROVIDER: GSE74041 | GEO | 2017-02-08

SECONDARY ACCESSION(S): PRJNA298852

REPOSITORIES: GEO

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Publications

Generation and Analysis of GATA2w/eGFP Human ESCs Reveal ITGB3/CD61 as a Reliable Marker for Defining Hemogenic Endothelial Cells during Hematopoiesis.

Huang Ke K   Gao Jiao J   Du Juan J   Ma Ning N   Zhu Yanling Y   Wu Pengfei P   Zhang Tian T   Wang Wenqian W   Li Yuhang Y   Chen Qianyu Q   Hutchins Andrew Paul AP   Yang Zhongzhou Z   Zheng Yi Y   Zhang Jian J   Shan Yongli Y   Li Xuejia X   Liao Baojian B   Liu Jiajun J   Wang Jinyong J   Liu Bing B   Pan Guangjin G  

Stem cell reports 20161013 5


The transition from hemogenic endothelial cells (HECs) to hematopoietic stem/progenitor cells (HS/PCs), or endothelial to hematopoietic transition (EHT), is a critical step during hematopoiesis. However, little is known about the molecular determinants of HECs due to the challenge in defining HECs. We report here the generation of GATA2w/eGFP reporter in human embryonic stem cells (hESCs) to mark cells expressing GATA2, a critical gene for EHT. We show that during differentiation, functional HEC  ...[more]

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