Project description:The current study aims to study change in gene expression profile of miltefosine resistance L. donovani with respect to miltefosine responsive L. donovani. Agilent one-color experiment,Organism: Leishmania donovani, Microarray GeneExpression 8x15k designed by Genotypic Technology Private Limited. (AMADID: 035638)
Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani. The antimony resistant and sensitive Leishmania donovani were grown in complete M199 medium with 10% FCS and Penicillin streptomycin mixture. At stationary phase (5 day culture) cells were harvested in sterile Phosphate buffered saline and used for RNA isolation.
Project description:Gene expression analysis was carried out between experimental miltefosine resistant L.donovani (adapted to 30ug/ml miltefosine drug pressure) and its corresponding wild type miltefosine senstive parasite 3 biological replicates of lab generated miltefosine resistant Leishmania donovani (K417 M30) were compared with corresponding wild type sensitive parasite (K417WT)
Project description:Murine bone marrow derived macrophages were infected with Leishmania major or Leishmania donovania promastigotes, allowed to phagocytose latex beads or not treated. Gene expression profiles were compared to identify i) the effect of Leishmania infection; ii) the differences in effects between L. major and L. donovani; and iii) the effect of pahgocytosis of latex beads.
Project description:In this study, we examined the transcriptome of Leishmania donovani promastigotes and axenic amastigotes to identify differentially regulated mRNAs utilizing the serial analysis of gene expression Keywords: stage differentiation; axenic amastigotes Overall design: Our approach was to identify Leishmania genes developmentally expressed during the promastigote and amastigote stages. Our assumption was that genes which expressed a difference between the promastigote and amastigote stages were likely to be essential to the transformation and the survival of the parasite within its human host.
Project description:Several intracellular pathogens target the host miRNA to modulate the expression of host proteins for their successful infection and survival. For example, S. typhimurium (Schulte et al., 2011) and Mycobacterium tuberculosis (Kumar et al., 2015) downregulate miRNAs of let-7 family to modulate immune response in host; H. pylori infection induces the expression of miR-155 to inhibit the release of IL-8 (Xiao et al., 2009); L. monocytogens triggers the expression of anti- inflammatory cytokine IFN-β by downregulation of miR-145 (Izar et al., 2012). Similarly, Leishmania infection also modulates the expression of various miRNA in macrophages (Lemaire et al., 2013). Consequently, it has been shown that L. donovani infection downregulates miR-122 expression which lowers serum cholesterol to facilitate infection (Ghosh et al., 2013). Whereas, Leishmania significantly enhances the miR30A- 3p expression and modulates autophagic pathway in macrophages (Singh et al., 2016). To understand how Leishmania modulate the expression of various genes in infected macrophages, we have compared the miRNA profile of uninfected and infected macrophages. Overall design: miRNA profiling of uninfected and Leishmania donovani infected THP1 differentiated macrophages after 15 h of infection was done to understand the mechanism of regulation of various genes.