Dataset Information


Metabolic inhibition of Clostridium thermocellum M1570 by pentose sugars.

ABSTRACT: Differential RNA-Seq analyses to investigate the basis for metabolic inhibition of Clostridium thermocellum M1570 by xylose. The M1570 strain was developed in the C. thermocellum DSM 1313 Δhpt background strain. Lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta) genes are deleted (Argyros DA, Tripathi SA, Barrett TF, Rogers SR, Feinberg LF, Olson DG, Foden JM, Miller BB, Lynd LR, Hogsett DA, Caiazza NC: High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes. Appl Environ Microbiol 2010, 77:8288-8294 ) Overall design: Differential RNA-Seq analyses was used to investigate the basis for metabolic inhibition of Clostridium thermocellum M1570 by xylose. Cultures were grown in serum bottles containing 40 mL of MTC medium with or without 15 g/L xylose and were harvested at mid-exponential phase. Quadruplicate cultures were used for each experimental condition tested.

INSTRUMENT(S): Illumina MiSeq (Ruminiclostridium thermocellum)

SUBMITTER: Dawn Marie Klingeman  

PROVIDER: GSE74918 | GEO | 2016-10-31



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Pentose sugars inhibit metabolism and increase expression of an AgrD-type cyclic pentapeptide in Clostridium thermocellum.

Verbeke Tobin J TJ   Giannone Richard J RJ   Klingeman Dawn M DM   Engle Nancy L NL   Rydzak Thomas T   Guss Adam M AM   Tschaplinski Timothy J TJ   Brown Steven D SD   Hettich Robert L RL   Elkins James G JG  

Scientific reports 20170223

Clostridium thermocellum could potentially be used as a microbial biocatalyst to produce renewable fuels directly from lignocellulosic biomass due to its ability to rapidly solubilize plant cell walls. While the organism readily ferments sugars derived from cellulose, pentose sugars from xylan are not metabolized. Here, we show that non-fermentable pentoses inhibit growth and end-product formation during fermentation of cellulose-derived sugars. Metabolomic experiments confirmed that xylose is t  ...[more]

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