Dataset Information


Transcriptomic responses of Clostridium (Ruminiclostridium) thermocellum ATCC27405 and ethanol tolerant strains to ethanol stress

ABSTRACT: Transcriptome profiles for Clostridium thermocellum ATCC 27405 wild type strain and two ethanol-adapted strains, E50A and E50C were generated to gain insights into ethanol tolerance. Details of the strains have been described, Shao X., et al. Appl Microbiol Biotechnol (2011) 92:641–652. Overall design: Duplicate fermentations using MTC medium were conducted for Clostridium thermocellum strains, ATCC 27405 wild-type; ethanol adapted strain E50A (adapted to grow in presence of up to 50 g/L ethanol on Avicel); and ethanol adapted strain E50C (adapted to grow in presence of up to 50 g/L ethanol on cellobiose). These strains have been described previously by Shao X., et al Appl Microbiol Biotechnol (2011) 92:641–652. A fifty two array study using total RNA processed from fermenation cultures of Clostridium thermocellum ATCC 27405 and ethanol adapted strains E50C and E50A, which were shocked with either 10 or 40 g/L ethanol. Cells were harvested immediately after, 15 minutes, 1 hour, 2 hours, and 4 hours after ethanol shock.

INSTRUMENT(S): NimbleGen Clostridium thermocellum ATCC 27405 HX12 array (12 x 135k)(non-condensed version) [080724_C_thermo_27405_expr]

SUBMITTER: Jason Whitham  

PROVIDER: GSE77089 | GEO | 2018-03-31


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