Transcriptomics,Genomics

Dataset Information

154

Single cell RNAseq of electrophysiologically characterized neurons of the hippocampus


ABSTRACT: Recent advances in single-cell RNAseq technologies are enabling new cell type classifications. For neurons, electrophysiological properties traditionally guide cell type classification but correlating RNAseq data with electrophysiological parameters has been difficult. Here we demonstrate RNAseq of electrophysiologically and synaptically characterized individual, patched neurons in the hippocampal CA1-region and subiculum, and relate the resulting transcriptome data to their electrical and synaptic properties. In this analysis, we explored the hypothesis that precise combinatorial interactions between matching cell-adhesion and signaling molecules shape synapse specificity. In analyzing interneurons and pyramidal neurons that are synaptically connected, we identified two independent, developmentally regulated networks of interacting genes encoding cell-adhesion, exocytosis and signal-transduction molecules. In this manner, our data allow postulating a presumed cell-adhesion and signaling code, which may explain neuronal connectivity at the molecular level. Our approach enables correlating electrophysiological with molecular properties of neurons, and suggests new avenues towards understanding synaptic specificity. Overall design: These data include 15 tissue samples (including 3 independent replicas in 5 developmental stages) as well as 93 single-cell samples (including CA1 cholecystokinin, parvalbumin, and pyramidal neurons as well as subiculum burst and regular firing pyramidal neurons).

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

SUBMITTER: Spyros Darmanis  

PROVIDER: GSE75386 | GEO | 2016-08-18

SECONDARY ACCESSION(S): PRJNA307566

REPOSITORIES: GEO

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Publications

Single-cell RNAseq reveals cell adhesion molecule profiles in electrophysiologically defined neurons.

Földy Csaba C   Darmanis Spyros S   Aoto Jason J   Malenka Robert C RC   Quake Stephen R SR   Südhof Thomas C TC  

Proceedings of the National Academy of Sciences of the United States of America 20160816 35


In brain, signaling mediated by cell adhesion molecules defines the identity and functional properties of synapses. The specificity of presynaptic and postsynaptic interactions that is presumably mediated by cell adhesion molecules suggests that there exists a logic that could explain neuronal connectivity at the molecular level. Despite its importance, however, the nature of such logic is poorly understood, and even basic parameters, such as the number, identity, and single-cell expression prof  ...[more]

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