Genomics

Dataset Information

40

Chronic binge alcohol administration dysregulates global regulatory gene networks associated with skeletal muscle wasting in simian immunodeficiency virus-infected macaques


ABSTRACT: Background: There are more than 1 million persons living with HIV/AIDS (PLWHA) in the United States and approximately 40% of them have a history of alcohol use disorders (AUD). Chronic heavy alcohol consumption and HIV/AIDS both result in reduced lean body mass and muscle dysfunction, increasing the incidence of comorbid conditions. Previous studies from our laboratory using rhesus macaques infected with Simian immunodeficiency Virus (SIV) demonstrated that chronic binge alcohol (CBA) administration in the absence of antiretroviral therapy exacerbates skeletal muscle (SKM) wasting at end-stage SIV disease. The aim of this study was to characterize how CBA alters global gene regulatory networks that lead to SKM wasting at end-stage disease. Administration of intragastric alcohol or sucrose to male rhesus macaques began 3 months prior to SIV infection and continued throughout the duration of study. High-output array analysis was used to determine CBA-dependent changes in mRNA expression, miRNA expression, and promoter methylation status of SKM at end-stage disease (~10 months post-SIV) from healthy control (control), sucrose-administered, SIV-infected (SUC/SIV), and CBA-administered/SIV-infected (CBA/SIV) macaques. Results: In addition to previously reported effects on the extracellular matrix and the promotion of a pro-inflammatory environment, we found that CBA adversely affects gene regulatory networks that involve universal cellular functions, protein homeostasis, calcium and ion homeostasis, neuronal growth and signaling, and satellite cell growth and survival. Conclusions: The results from this study provide an overview of the impact of CBA on gene regulatory networks involved in biological functions, including transcriptional and epigenetic processes, illustrating the genetic and molecular mechanisms associated with CBA-dependent SKM wasting at end-stage SIV infection. Overall design: Animals were exposed to daily intragastric administration of a mean of 2.5 g per kg body weight ethanol (30% w/v water), beginning 3 months prior to SIV infection and continuing throughout the duration of study. Three months later animals were inoculated intravenously with 10,000 times the 50% infective dose (ID50) of SIVmac251. SKM tissues were collected and snap frozen until use.

INSTRUMENT(S): Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)

SUBMITTER: Jovanny Zabaleta  

PROVIDER: GSE75729 | GEO | 2015-12-17

SECONDARY ACCESSION(S): PRJNA305200

REPOSITORIES: GEO

altmetric image

Publications

Chronic binge alcohol administration dysregulates global regulatory gene networks associated with skeletal muscle wasting in simian immunodeficiency virus-infected macaques.

Simon Liz L   Hollenbach Andrew D AD   Zabaleta Jovanny J   Molina Patricia E PE  

BMC genomics 20151223


There are more than 1 million persons living with HIV/AIDS (PLWHA) in the United States and approximately 40 % of them have a history of alcohol use disorders (AUD). Chronic heavy alcohol consumption and HIV/AIDS both result in reduced lean body mass and muscle dysfunction, increasing the incidence of comorbid conditions. Previous studies from our laboratory using rhesus macaques infected with Simian Immunodeficiency Virus (SIV) demonstrated that chronic binge alcohol (CBA) administration in the  ...[more]

Similar Datasets

| GSE69685 | GEO
2019-01-01 | S-EPMC6766701 | BioStudies
2019-01-01 | S-EPMC6751413 | BioStudies
2015-01-01 | S-EPMC4691397 | BioStudies
| GSE59111 | GEO
1000-01-01 | S-EPMC4396606 | BioStudies
1000-01-01 | S-EPMC191219 | BioStudies
2015-01-01 | S-EPMC4300675 | BioStudies
1000-01-01 | S-EPMC2111118 | BioStudies
2013-01-01 | S-EPMC3812314 | BioStudies