Genomics

Dataset Information

0

Ofr1 regulates white-to-opaque switching and mating of Candida albicans (MTLa/α)


ABSTRACT: It has been proposed that the ancestral fungus was mating competent and homothallic. However, many mating competent fungi were initially classified as asexual because their mating capacity was hidden behind layers of regulation. For efficient in vitro mating, the essentially obligate diploid ascomycete pathogen C. albicans has to homozygose its mating type locus from MTLa/α to MTLa/a or MTLα/α, and then undergo an environmentally controlled epigenetic switch to the mating competent opaque form. These requirements greatly reduce the potential for C. albicans mating. Deletion of the YciI domain gene OFR1 bypasses the need for C. albicans cells to homozygose the mating type locus prior to switching to the opaque form and mating, and allows homothallic mating of MTL heterozygous strains. This bypass is carbon source dependent and does not occur when cells are grown on glucose. Transcriptional profiling of ofr1 mutant cells shows that in addition to regulating cell type and mating circuitry, Ofr1 is needed for proper regulation of histone and chitin biosynthesis gene expression. It appears that OFR1 is a key regulator in C. albicans, and functions in part to maintain the cryptic mating phenotype of the pathogen. Distruption of OFR1 gene which encodes a Yci1 related domain in Candida ablicans (MTLa/α) is shown to have white-opaque switching related and mating related gene expression. The expression of histone genes are also positively regulated in some conditions.

ORGANISM(S): Candida albicans

PROVIDER: GSE75780 | GEO | 2015/12/29

SECONDARY ACCESSION(S): PRJNA305349

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-43938 | biostudies-arrayexpress
2015-12-28 | GSE74011 | GEO
2015-12-30 | GSE67101 | GEO
2014-10-23 | GSE56039 | GEO
| E-GEOD-56039 | biostudies-arrayexpress
2004-12-01 | GSE1969 | GEO
2023-03-03 | PXD040559 | iProX
2013-04-01 | GSE43938 | GEO
2007-10-01 | GSE8709 | GEO
| PRJNA305349 | ENA