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RNA-Seq data for KRAS wild-type(WT) and two mutant forms G12V and Q61H over-expressed samples with green fluorescent protein control samples using human mammary epithelial cells

ABSTRACT: The goal was to capture the transcriptional activity due to over-expression of KRAS gene. Over-expressions were validated using Western Blots. Illumina RNA-Seq technology was used to capture the downstream transcriptional activity. Reads were 101 base pairs long and single ended. An R open source package “Rsubread” was used to align and quantify the read using UCSC hg19 annotation. The integer-based gene counts were later normalized in TPM .

ORGANISM(S): Homo sapiens

PROVIDER: GSE76877 | GEO | 2017/05/01

SECONDARY ACCESSION(S): PRJNA308839

REPOSITORIES: GEO

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A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms.